Optoacoustic imaging of glucagon-like peptide-1 receptor with a near-infrared exendin-4 analog Journal Article
| Authors: | Roberts, S.; Khera, E.; Choi, C.; Navaratna, T.; Grimm, J.; Thurber, G. M.; Reiner, T. |
| Article Title: | Optoacoustic imaging of glucagon-like peptide-1 receptor with a near-infrared exendin-4 analog |
| Abstract: | Limitations in current imaging tools have long challenged the imaging of small pancreatic islets in animal models. Here, we report the first development and in vivo validation testing of a broad-spectrum and high-absorbance near-infrared optoacoustic contrast agent, E4x12-Cy7. Our near-infrared tracer is based on the amino acid sequence of exendin-4 and targets the glucagon-like peptide-1 receptor (GLP-1R). Cell assays confirmed that E4x12-Cy7 has a high-binding affinity (dissociation constant, Kd, 4.66 0.8 nM). Using themultispectral optoacoustic tomography, we imaged E4x12-Cy7 and optoacoustically visualized b-cell insulinoma xenografts in vivo for the first time. In the future, similar optoacoustic tracers that are specific for b-cells and combines optoacoustic and fluorescence imaging modalities could prove to be important tools for monitoring the pancreas for the progression of diabetes. © 2021 Society of Nuclear Medicine Inc.. All rights reserved. |
| Keywords: | diabetes; near infrared; sonophore; msot; b-cell imaging; exendin- 4; optoacoustic |
| Journal Title: | Journal of Nuclear Medicine |
| Volume: | 62 |
| Issue: | 6 |
| ISSN: | 0161-5505 |
| Publisher: | Society of Nuclear Medicine |
| Date Published: | 2021-06-01 |
| Start Page: | 839 |
| End Page: | 848 |
| Language: | English |
| DOI: | 10.2967/jnumed.120.252262 |
| PUBMED: | 33097631 |
| PROVIDER: | scopus |
| PMCID: | PMC8729860 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 July 2021 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work