Embryonic lethal abnormal vision-like RNA-binding proteins regulate neurite outgrowth and tau expression in PC12 cells Journal Article
| Authors: | Aranda-Abreu, G. E.; Behar, L.; Chung, S.; Furneaux, H.; Ginzburg, I. |
| Article Title: | Embryonic lethal abnormal vision-like RNA-binding proteins regulate neurite outgrowth and tau expression in PC12 cells |
| Abstract: | The embryonic lethal abnormal vision (ELAV)-like proteins are mRNA- binding proteins that regulate mRNA stability. The neuronal members of this family are required for neuronal differentiation. We identified the binding region of purified HuD protein to a target neuronal mRNA encoding for the tau microtubule-associated protein and demonstrated an in vivo interaction between the ELAV-like protein and its target tau mRNA. We show that treatment of neuronal cells with antisense oligodeoxynucleotides directed against HuD blocks the induction of neurite outgrowth and decreases the levels of tau mRNAs, indicating that the ELAV-like proteins are required for neuronal differentiation. |
| Keywords: | controlled study; protein expression; nonhuman; animal cell; animals; reverse transcription polymerase chain reaction; nerve tissue proteins; protein stability; cell differentiation; neurons; rna binding protein; rna-binding proteins; molecular sequence data; cell culture; messenger rna; reverse transcriptase polymerase chain reaction; rna, messenger; nucleotide sequence; rat; immunoprecipitation; base sequence; cell polarity; cell fractionation; rats; microtubule; microtubules; molecular weight; hu paraneoplastic encephalomyelitis antigens; oligonucleotides, antisense; tau protein; gel electrophoresis; caenorhabditis elegans proteins; neurite outgrowth; antisense oligodeoxynucleotide; nerve fiber growth; tau proteins; pc12 cells; neurites; mrna stabilization; antisense oligodeoxynucleotides; humans; priority journal; article; tau mrna; pheochromocytoma cell |
| Journal Title: | The Journal of Neuroscience |
| Volume: | 19 |
| Issue: | 16 |
| ISSN: | 0270-6474 |
| Publisher: | Society for Neuroscience |
| Date Published: | 1999-08-15 |
| Start Page: | 6907 |
| End Page: | 6917 |
| Language: | English |
| PUBMED: | 10436048 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
