Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein Journal Article

  


Authors: Ma, W. J.; Cheng, S.; Campbell, C.; Wright, A.; Furneaux, H.
Article Title: Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein
Abstract: The neuronal-specific Elav-like proteins (HuD, Hel-N, and HuC) contain three RNP-type concensus motifs and bind to AU-rich elements. We have identified and cloned a fourth member of this family (HuR) that is expressed in a wide variety of cell types. The purified recombinant protein binds avidly to the AU-rich element in c-fos and interleukin-3 mRNAs. In the case of the c-fos AU-rich element, HuR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Mutational analysis has shown that all three AU motifs are required for maximal binding.
Keywords: gene mutation; human cell; gene expression regulation; molecular cloning; cloning, molecular; rna-binding proteins; amino acid sequence; molecular sequence data; sequence homology, amino acid; messenger rna; rna, messenger; sequence alignment; nucleotide sequence; genes, myc; base sequence; binding sites; dna primers; antigens, surface; dna, complementary; protein synthesis regulation; interleukin-3; humans; human; priority journal; article; genes, fos
Journal Title: Journal of Biological Chemistry
Volume: 271
Issue: 14
ISSN: 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology  
Date Published: 1996-04-05
Start Page: 8144
End Page: 8151
Language: English
DOI: 10.1074/jbc.271.14.8144
PUBMED: 8626503
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029873536&doi=10.1074%2fjbc.271.14.8144&partnerID=40&md5=e74cb6e8898191ed844c1e7ea1c66a0b
Notes: Article -- Export Date: 22 November 2017 -- Source: Scopus
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MSK Authors
  1. Henry M Furneaux
    57 Furneaux
  2. Wei-Jun Ma
    6 Ma
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