The Elav-like proteins bind to AU-rich elements and to the poly(A) tail of mRNA Journal Article
| Authors: | Ma, W. J.; Chung, S.; Furneaux, H. |
| Article Title: | The Elav-like proteins bind to AU-rich elements and to the poly(A) tail of mRNA |
| Abstract: | The Elav-like proteins are specific mRNA binding proteins which are required for cellular differentiation. They contain three characteristic RNP2/RNP1-type RNA binding motifs. Previously we have shown that the first and second RNA binding domains bind to AU-rich elements in the 3'-UTR of mRNA. In this paper we show that the Elav-like proteins exhibit poly(A) binding activity. This activity is distinct from poly(A) binding activities that have been previously described. The Elav-like proteins specifically bind to long chain poly(A) tails. We have shown that the third RNA binding domain encompasses this poly(A) binding activity. Using poly(A)-Sepharose beads in a 'sandwich' assay we have shown that the Elav-like proteins can bind simultaneously to the AU-rich element and to the poly(A) tail. |
| Keywords: | controlled study; sequence analysis; protein domain; cell differentiation; rna binding protein; rna-binding proteins; molecular sequence data; messenger rna; rna, messenger; ribonucleoproteins; base sequence; binding sites; hu paraneoplastic encephalomyelitis antigens; protein rna binding; polyadenylated rna; priority journal; article |
| Journal Title: | Nucleic Acids Research |
| Volume: | 25 |
| Issue: | 18 |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Date Published: | 1997-09-01 |
| Start Page: | 3564 |
| End Page: | 3569 |
| Language: | English |
| DOI: | 10.1093/nar/25.18.3564 |
| PUBMED: | 9278474 |
| PROVIDER: | scopus |
| PMCID: | PMC146929 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
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