Cancer-testis antigens and ING1 tumor suppressor gene product are breast cancer antigens: Characterization of tissue-specific ING1 transcripts and a homologue gene Journal Article
| Authors: | Jäger, D.; Stockert, E.; Scanlan, M. J.; Güre, A. O.; Jäger, E.; Knuth, A.; Old, L. J.; Chen, Y. T. |
| Article Title: | Cancer-testis antigens and ING1 tumor suppressor gene product are breast cancer antigens: Characterization of tissue-specific ING1 transcripts and a homologue gene |
| Abstract: | SEREX (serological analysis of recombinant tumor cDNA expression libraries) has been applied to several different tumor types and has led to the identification of a wide range of tumor antigens. In this study, a breast cancer library and a normal testicular library were analyzed using autologous and allogeneic breast cancer sera. Thirty genes were isolated, including 27 known genes and 3 previously unknown genes. Among the known genes, two cancer-testis (CT) antigens, NY-ESO-1 and SSX2, previously defined by SEREX analysis, were found. In addition, ING1, a candidate breast cancer suppressor gene, was isolated. This ING1 gene product was also recognized by 2 of 14 allogeneic sera from breast cancer patients but not 12 normal adult sera. Comparison of ING1 cDNA from normal and tumor tissues showed no mutation in the index breast cancer case and revealed the presence of at least three different mRNA transcripts with variable transcription initiation sites and exon usage. Tissue-specific expression of these transcripts was found in normal tissues and tumor cell line mRNAs. Furthermore, a novel gene, designated as ING2, sharing 76% nucleotide homology with ING1 was identified in the breast cancer cDNA library. The basis of the immunogenicity of ING1 and the biological role of ING1 and ING2 need further exploration. |
| Keywords: | gene sequence; human cell; sequence analysis; exon; mutation; dna-binding proteins; proteins; cell cycle proteins; breast cancer; gene expression; transcription initiation; neoplasm proteins; membrane proteins; gene product; genetic transcription; tumor cells, cultured; gene library; breast neoplasms; tumor antigen; nuclear proteins; cloning, molecular; amino acid sequence; molecular sequence data; sequence homology, amino acid; antigens, neoplasm; tissue distribution; messenger rna; rna, messenger; intracellular signaling peptides and proteins; nucleotide sequence; tumor suppressor proteins; alternative splicing; base sequence; sequence homology; sequence homology, nucleic acid; repressor proteins; testis; genes, tumor suppressor; rna transcription; humans; human; male; female; priority journal; article |
| Journal Title: | Cancer Research |
| Volume: | 59 |
| Issue: | 24 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1999-12-15 |
| Start Page: | 6197 |
| End Page: | 6204 |
| Language: | English |
| PUBMED: | 10626813 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
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