Characterization of human colon cancer antigens recognized by autologous antibodies Journal Article


Authors: Scanlan, M. J.; Chen, Y. T.; Williamson, B.; Gure, A. O.; Stockert, E.; Gordan, J. D.; Türeci, Ö; Sahin, U.; Pfrendschuh, M.; Old, L. J.
Article Title: Characterization of human colon cancer antigens recognized by autologous antibodies
Abstract: The screening of cDNA expression libraries derived from human tumors with autologous antibody (SEREX) has proven to be a powerful method for defining the structure of tumor antigens recognized by the humoral immune system. In the present study, 48 distinct antigens (NY-CO-I-NY-CO-48)reactive with autologous IgG were identified by SEREX analysis in 4 patients with colon cancer. Sequencing analysis showed that 17 of the cDNA clones were previously uncharacterized molecules and 31 represented known gene products. The individual cDNA clones were analyzed in the following manner: a search for mutations or other structural changes; an analysis of mRNA expression in a panel of normal tissues; and a frequency analysis of the antibody response to the expressed product in the sera of colon cancer patients and normal individuals. The initial analysis showed NY-CO-13 to be a mutated version of the p53 tumor suppressor gene. Three of the 48 antigens showed a differential pattern of mRNA expression, with NY-CO-27 (galectin-4) expressed primarily in gastrointestinal tract, and NY-CO-37 and -38 showing a pattern of tissue- specific isoforms. With regard to immunogenicity, 20 of the 48 antigens were detected by allogeneic sera; 14 of these were reactive with sera from both normal donors and cancer patients, and 6 other clones (NY-CO-8, -9, -13, - 16, -20 and -38) reacted exclusively with sera from colon cancer patients (ranging from 14% to 27%). Our results on colon cancer illustrate both the complexity and the potential of the SEREX approach for analysis of the humoral immune response against human cancer.
Keywords: adult; human tissue; gene mutation; colonic neoplasms; tumor antigen; molecular cloning; cloning, molecular; cancer genetics; tumor suppressor gene; immune response; antigens, neoplasm; immunoglobulin g; rna, messenger; colon cancer; immunogenicity; nucleotide sequence; antigen recognition; dna, neoplasm; dna sequence; unindexed sequence; autoantibodies; antibodies, neoplasm; antibody; humans; human; male; female; priority journal; article
Journal Title: International Journal of Cancer
Volume: 76
Issue: 5
ISSN: 0020-7136
Publisher: John Wiley & Sons  
Date Published: 1998-05-29
Start Page: 652
End Page: 658
Language: English
DOI: 10.1002/(sici)1097-0215(19980529)76:5<652::aid-ijc7>3.0.co;2-p
PUBMED: 9610721
PROVIDER: scopus
DOI/URL:
Notes: Article -- Export Date: 12 December 2016 -- Source: Scopus
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  1. Matthew J Scanlan
    49 Scanlan
  2. Ali O Gure
    29 Gure
  3. Lloyd J Old
    593 Old
  4. Yao-Tseng Chen
    83 Chen