Identification of breast cancer-restricted antigens by antibody screening of SKBR3 cDNA library using a preselected patient's serum Journal Article
| Authors: | Forti, S.; Scanlan, M. J.; Invernizzi, A.; Castiglioni, F.; Pupa, S.; Agresti, R.; Fontanelli, R.; Morelli, D.; Old, L. J.; Pupa, S. M.; Ménard, S. |
| Article Title: | Identification of breast cancer-restricted antigens by antibody screening of SKBR3 cDNA library using a preselected patient's serum |
| Abstract: | Screening of a breast cancer cDNA library from SKBR3 human breast cancer cells by SEREX (serological analysis of cDNA expression library) using a preselected serum from a breast cancer patient revealed 13 genes, two of which, INT-MI-1 and INT-MI-2, encode novel gene products, while the remaining 11 genes and their products are identical with or highly homologous to known GenBank entries. Immunoscreening of the 13 clones using 20 allogeneic sera from breast cancer patients and 20 samples from age- and gender-matched healthy donors showed that lactate dehydrogenase-A (LDH-A), lactate dehydrogenase-B (LDH-B), fibulin-1, and thyroid hormone-binding protein (THBP) were recognized principally by the breast cancer patient sera, indicating the immunogenicity of these molecules in vivo. The other antigens were similarly recognized by normal and patients sera, and thus not tumor-restricted immunologically. RT-PCR analysis revealed strong expression of fibulin-1 in tumor cell lines and surgical specimen whereas in the same experimental conditions, normal tissues scored negative. Also THBP expression was found in various tumors whereas in normal tissues, its expression is restricted to the testis and, at lower levels, in ovary, liver, and spleen. In contrast, LDH-A and LDH-B were ubiquitously expressed in normal and tumor tissues, with LDH-B levels considerably lower and heterogeneous in normal samples compared to those expressed in tumor cell lines. The differential expression of fibulin-1 between the normal tissues and breast carcinoma cell lines (5/6) and surgical specimens (5/6) suggests the possible involvement of the overexpression of this extracellular matrix-associated glycoprotein in the pathogenesis of this neoplasm. |
| Keywords: | controlled study; human tissue; human cell; genetics; cancer patient; gene overexpression; reverse transcription polymerase chain reaction; breast cancer; gene expression; mass screening; gene product; cancer cell culture; pathology; tumor cells, cultured; gene library; breast neoplasms; tumor antigen; biopsy; scleroprotein; gene expression regulation; gene expression regulation, neoplastic; biosynthesis; immunology; immunotherapy; antigens; antigens, neoplasm; cell culture; reverse transcriptase polymerase chain reaction; immunogenicity; nucleotide sequence; breast tumor; breast carcinoma; carcinoma; tumor immunity; lactate dehydrogenase; calcium binding protein; calcium-binding proteins; autoantibody; autoantibodies; thyroid hormone; antibody screening; clone; fibulin; dna library; humans; human; female; priority journal; article; support, non-u.s. gov't; immunome; hormone binding protein; genbank |
| Journal Title: | Breast Cancer Research and Treatment |
| Volume: | 73 |
| Issue: | 3 |
| ISSN: | 0167-6806 |
| Publisher: | Springer |
| Date Published: | 2002-06-01 |
| Start Page: | 245 |
| End Page: | 256 |
| Language: | English |
| DOI: | 10.1023/a:1015854415746 |
| PUBMED: | 12160330 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
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