Fluorescence in situ hybridization analysis of circulating tumor cells in metastatic prostate cancer Journal Article


Authors: Leversha, M. A.; Han, J.; Asgari, Z.; Danila, D. C.; Lin, O.; Gonzalez-Espinoza, R.; Anand, A.; Lilja, H.; Heller, G.; Fleisher, M.; Scher, H. I.
Article Title: Fluorescence in situ hybridization analysis of circulating tumor cells in metastatic prostate cancer
Abstract: Purpose: To assess the feasibility of characterizing gene copy number alteration by fluorescence in situ hybridization (FISH) of circulating tumor cells (CTC) isolated using the Cell Search system in patients with progressive castration-resistant metastatic prostate cancer. Experimental Design: We used probe combinations that included the androgen receptor (AR)and MYC genes for FISH analysis of CTC samples collected from 77 men with castration-resistant metastatic prostate cancer. Results: High-level chromosomal amplification of AR was detected in 38% and relative gain of MYC in 56% of samples analyzed. No such abnormalities were detected in samples with CTC counts of <10, reflecting ascertainment difficulty in these lower count samples. Conclusion: The CTC isolated from our patient cohort present a very similar molecular cytogenetic profile to that reported for late-stage tumors and show that FISH analysis of CTC can be a valuable, noninvasive surrogate for routine tumor profiling. That as many as 50% of these patients have substantial amplification of the AR locus indicates that androgen signaling continues to play an important role in late-stage prostate cancer. © 2009 American Association for Cancer Research.
Keywords: major clinical study; cancer staging; in situ hybridization, fluorescence; metastasis; gene amplification; prostate cancer; prostatic neoplasms; gene number; fluorescence in situ hybridization; neoplastic cells, circulating; genes, myc; tumor cell; androgen receptor; castration; receptors, androgen; gene dosage; chromosome analysis; oncogene myc
Journal Title: Clinical Cancer Research
Volume: 15
Issue: 6
ISSN: 1078-0432
Publisher: American Association for Cancer Research  
Date Published: 2009-03-15
Start Page: 2091
End Page: 2097
Language: English
DOI: 10.1158/1078-0432.ccr-08-2036
PUBMED: 19276271
PROVIDER: scopus
PMCID: PMC2875199
DOI/URL:
Notes: --- - "Cited By (since 1996): 16" - "Export Date: 30 November 2010" - "CODEN: CCREF" - "Source: Scopus"
Altmetric Score
MSK Authors
  1. Glenn Heller
    291 Heller
  2. Oscar Lin
    231 Lin
  3. Hans Gosta Lilja
    286 Lilja
  4. Martin Fleisher
    236 Fleisher
  5. Howard Scher
    816 Scher
  6. Daniel C Danila
    82 Danila
  7. Aseem Anand
    45 Anand
  8. Jialian Han
    1 Han
  9. Zahra Asgari
    6 Asgari