Circulating tumor cell analysis in patients with progressive castration-resistant prostate cancer Journal Article
| Authors: | Shaffer, D. R.; Leversha, M. A.; Danila, D. C.; Lin, O.; Gonzalez-Espinoza, R.; Gu, B.; Anand, A.; Smith, K.; Maslak, P.; Doyle, G. V.; Terstappen, L. W. M. M.; Lilja, H.; Heller, G.; Fleisher, M.; Scher, H. I. |
| Article Title: | Circulating tumor cell analysis in patients with progressive castration-resistant prostate cancer |
| Abstract: | Purpose: To better direct targeted therapies to the patients with tumors that express the target, there is an urgent need for blood-based assays that provide expression information on a consistent basis in real time with minimal patient discomfort. We aimed to use immunomagnetic-capture technology to isolate and analyze circulating tumor cells (CTC) from small volumes of peripheral blood of patients with advanced prostate cancer. Experimental Design: Blood was collected from 63 patients with metastatic prostate cancer. CTCs were isolated by the CellSearch system, which uses antibodies to epithelial cell adhesion marker and immunomagnetic capture. CTCs were defined as nucleated cells positive for cytokeratins and negative for CD45. Captured cells were analyzed by immunofluorescence, Papanicolau staining, and fluorescence in situ hybridization. Results: Most patients (65%) had 5 or more CTCs per 7.5 mL blood sample. Cell counts were consistent between laboratories (c = 0.99) and did not change significantly over 72 or 96 h of storage before processing (c = 0.99). Their identity as prostate cancer cells was confirmed by conventional cytologic analysis. Molecular profiling, including analysis of epidermal growth factor receptor (EGFR) expression, chromosome ploidy, and androgen receptor (AR) gene amplification, was possible for all prostate cancer patients with ≥5 CTCs. Conclusions: The analysis of cancer-related alterations at the DNA and protein level from CTCs is feasible in a hospital-based clinical laboratory. The alterations observed in EGFR and AR suggest that the methodology may have a role in clinical decision making. © 2007 American Association for Cancer Research. |
| Keywords: | adult; controlled study; protein expression; aged; aged, 80 and over; middle aged; gene mutation; human cell; advanced cancer; flow cytometry; reproducibility; prostate specific antigen; reproducibility of results; in situ hybridization, fluorescence; metastasis; gene amplification; epidermal growth factor receptor; receptor, epidermal growth factor; prostate cancer; prostatic neoplasms; fluorescent antibody technique; fluorescence in situ hybridization; immunocytochemistry; immunomagnetic separation; tumor cell; cell count; androgen receptor; receptor, erbb-2; epithelial cell adhesion molecule; castration; receptors, androgen; cytokeratin; cytopathology; cd45 antigen; cytological techniques; papanicolaou test; phlebotomy; neoplasm circulating cells; ploidy; laboratory automation |
| Journal Title: | Clinical Cancer Research |
| Volume: | 13 |
| Issue: | 7 |
| ISSN: | 1078-0432 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 2007-04-01 |
| Start Page: | 2023 |
| End Page: | 2029 |
| Language: | English |
| DOI: | 10.1158/1078-0432.ccr-06-2701 |
| PUBMED: | 17404082 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 95" - "Export Date: 17 November 2011" - "CODEN: CCREF" - "Source: Scopus" |
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