The tumor suppressor PTEN and the PDK1 kinase regulate formation of the columnar neural epithelium Journal Article

Authors: Grego Bessa, J.; Bloomekatz, J.; Castel, P.; Omelchenko, T.; Baselga, J.; Anderson, K. V.
Article Title: The tumor suppressor PTEN and the PDK1 kinase regulate formation of the columnar neural epithelium
Abstract: Epithelial morphogenesis and stability are essential for normal development and organ homeostasis. The mouse neural plate is a cuboidal epithelium that remodels into a columnar pseudostratified epithelium over the course of 24 hr. Here we show that the transition to a columnar epithelium fails in mutant embryos that lack the tumor suppressor PTEN, although proliferation, patterning and apical-basal polarity markers are normal in the mutants. The Pten phenotype is mimicked by constitutive activation of PI3 kinase and is rescued by the removal of PDK1 (PDPK1), but does not depend on the downstream kinases AKT and mTORC1. High resolution imaging shows that PTEN is required for stabilization of planar cell packing in the neural plate and for the formation of stable apical-basal microtubule arrays. The data suggest that appropriate levels of membrane-associated PDPK1 are required for stabilization of apical junctions, which promotes cell elongation, during epithelial morphogenesis. © Grego-Bessa et al.
Keywords: protein expression; nonhuman; cell proliferation; mouse; embryo; phosphatidylinositol 3 kinase; in situ hybridization; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase; beta galactosidase; immunoblotting; cell shape; microtubule; cell elongation; phosphoinositide dependent protein kinase 1; neural tube defect; neuroectoderm; scanning electron microscopy; mammalian target of rapamycin complex 1; article
Journal Title: eLife
Volume: 5
ISSN: 2050-084X
Publisher: eLife Sciences Publications Ltd.  
Date Published: 2016-01-26
Start Page: e12034
Language: English
DOI: 10.7554/eLife.12034
PROVIDER: scopus
PMCID: PMC4739759
PUBMED: 26809587
Notes: Article -- Export Date: 3 March 2016 -- Source: Scopus
Citation Impact
MSK Authors
  1. Kathryn Anderson
    147 Anderson
  2. Pau Castel
    25 Castel
  3. Jose T Baselga
    483 Baselga