Functional cross-talk between Cdc42 and two downstream targets, Par6B and PAK4 Journal Article

Authors: Jin, D.; Durgan, J.; Hall, A.
Article Title: Functional cross-talk between Cdc42 and two downstream targets, Par6B and PAK4
Abstract: The establishment of polarity is an essential step in epithelial morphogenesis. Polarity proteins promote an apical/basal axis, which, together with the assembly of apical adherens and tight junctions, directed vesicle transport and the reorganization of the actomyosin filament network, generate a stable epithelium. The regulation of these cellular activities is complex, but the Rho family GTPase Cdc42 (cell division cycle 42) is known to play a key role in the establishment of polarity from yeast to humans. Two Cdc42 target proteins, the kinase PAK4 [p21 protein (Cdc42/Rac)-activated kinase 4] and the scaffold partitioning defective (Par) 6B, are required to promote the assembly of apical junctions in human bronchial epithelial cells. We show in the present paper that PAK4 phosphorylates Par6B at Ser143 blocking its interaction with Cdc42. This provides a potential new mechanism for controlling the subcellular localization of Par6B and its interaction with other proteins.
Keywords: genetics; mouse; animal; cytology; metabolism; animals; mice; cell line; phosphorylation; physiology; protein transport; epithelium cell; adaptor proteins, signal transducing; epithelial cells; cell polarity; signal transducing adaptor protein; p21 activated kinase; protein cdc42; p21-activated kinases; cdc42 gtp-binding protein; humans; human; cdc42 protein, mouse; pak4 protein, human; pak4 protein, mouse; par6b protein, human
Journal Title: Biochemical Journal
Volume: 467
Issue: 2
ISSN: 0264-6021
Publisher: Portland Press Ltd  
Date Published: 2015-04-15
Start Page: 293
End Page: 302
Language: English
DOI: 10.1042/bj20141352
PUBMED: 25662318
PROVIDER: scopus
Notes: Export Date: 2 July 2015 -- Source: Scopus
Citation Impact
MSK Authors
  1. Alan Hall
    41 Hall
  2. Dan Jin
    4 Jin