Cdc42 controls spindle orientation to position the apical surface during epithelial morphogenesis Journal Article

Authors: Jaffe, A. B.; Kaji, N.; Durgan, J.; Hall, A.
Article Title: Cdc42 controls spindle orientation to position the apical surface during epithelial morphogenesis
Abstract: The establishment of apical-basal polarity within a single cell and throughout a growing tissue is a key feature of epithelial morphogenesis. To examine the underlying mechanisms, the human intestinal epithelial cell line Caco-2 was grown in a three-dimensional matrix to generate a cystlike structure, where the apical surface of each epithelial cell faces a fluid-filled central lumen. A discrete apical domain is established as early as the first cell division and between the two daughter cells. During subsequent cell divisions, the apical domain of each daughter cell is maintained at the center of the growing structure through a combination of mitotic spindle orientation and asymmetric abscission. Depletion of Cdc42 does not prevent the establishment of apical-basal polarity in individual cells but rather disrupts spindle orientation, leading to inappropriate positioning of apical surfaces within the cyst. We conclude that Cdc42 regulates epithelial tissue morphogenesis by controlling spindle orientation during cell division. © 2008.
Keywords: controlled study; human tissue; human cell; nonhuman; animal cell; cytology; metabolism; cell division; cell structure; cell growth; protein depletion; cell line; morphogenesis; daughter cell; physiology; regulatory mechanism; epithelium cell; epithelial cells; intestine epithelium cell; cell polarity; spindle cell; mitosis spindle; mitotic spindle apparatus; liquid; caco-2 cells; intestine; protein cdc42; abscission; cyst; cell surface; cell strain caco 2; cdc42 gtp-binding protein
Journal Title: Journal of Cell Biology
Volume: 183
Issue: 4
ISSN: 0021-9525
Publisher: Rockefeller University Press  
Date Published: 2008-11-17
Start Page: 625
End Page: 633
Language: English
DOI: 10.1083/jcb.200807121
PUBMED: 19001128
PROVIDER: scopus
PMCID: PMC2582895
Notes: --- - "Cited By (since 1996): 51" - "Export Date: 17 November 2011" - "CODEN: JCLBA" - "Source: Scopus"
Citation Impact
MSK Authors
  1. Aron B Jaffe
    4 Jaffe
  2. Alan Hall
    41 Hall
  3. Joanne Durgan
    8 Durgan
  4. Noriko Kaji
    2 Kaji