Morphogenesis of the mouse neural plate depends on distinct roles of cofilin 1 in apical and basal epithelial domains Journal Article

Authors: Grego Bessa, J.; Hildebrand, J.; Anderson, K. V.
Article Title: Morphogenesis of the mouse neural plate depends on distinct roles of cofilin 1 in apical and basal epithelial domains
Abstract: The genetic control of mammalian epithelial polarity and dynamics can be studied in vivo at cellular resolution during morphogenesis of the mouse neural tube. The mouse neural plate is a simple epithelium that is transformed into a columnar pseudostratified tube over the course of ∼24 h. Apical F-actin is known to be important for neural tube closure, but the precise roles of actin dynamics in the neural epithelium are not known. To determine how the organization of the neural epithelium and neural tube closure are affected when actin dynamics are blocked, we examined the cellular basis of the neural tube closure defect in mouse mutants that lack the actin-severing protein cofilin 1 (CFL1). Although apical localization of the adherens junctions, the Par complex, the Crumbs complex and SHROOM3 is normal in the mutants, CFL1 has at least two distinct functions in the apical and basal domains of the neural plate. Apically, in the absence of CFL1 myosin light chain does not become phosphorylated, indicating that CFL1 is required for the activation of apical actomyosin required for neural tube closure. On the basal side of the neural plate, loss of CFL1 has the opposite effect on myosin: excess F-actin and myosin accumulate and the ectopic myosin light chain is phosphorylated. The basal accumulation of F-actin is associated with the assembly of ectopic basal tight junctions and focal disruptions of the basement membrane, which eventually lead to a breakdown of epithelial organization. © 2015. Published by The Company of Biologists Ltd.
Keywords: immunohistochemistry; controlled study; nonhuman; protein function; protein localization; mitosis; animal cell; mouse; phenotype; mammalia; actin; amino acid substitution; basement membrane; embryo; embryo development; morphogenesis; mixed lymphocyte culture; nerve cell adhesion molecule; western blotting; cell migration; cell count; cell shape; cell junction; f actin; beta catenin; myosin adenosine triphosphatase; depolymerization; apical membrane; neural tube; null allele; centrosome; neural tube defect; transmission electron microscopy; neuroectoderm; scanning electron microscopy; protein zo1; surface area; cofilin; epithelial polarity; neuroepithelium; cofilin 1; female; priority journal; article; epithelium basal cell; shroom3; mouse genetics; protein zo2
Journal Title: Development
Volume: 142
Issue: 7
ISSN: 0950-1991
Publisher: Company of Biologists  
Date Published: 2015-04-01
Start Page: 1305
End Page: 1314
Language: English
DOI: 10.1242/dev.115493
PROVIDER: scopus
PMCID: PMC4378245
PUBMED: 25742799
Notes: Export Date: 2 July 2015 -- Source: Scopus
Citation Impact
MSK Authors
  1. Kathryn Anderson
    148 Anderson