T311 - An anti-tyrosinase monoclonal antibody for the detection of melanocytic lesions in paraffin embedded tissues Journal Article


Authors: Jungbluth, A. A.; Iversen, K.; Coplan, K.; Kolb, D.; Stockert, E.; Chen, Y. T.; Old, L. J.; Busam, K.
Article Title: T311 - An anti-tyrosinase monoclonal antibody for the detection of melanocytic lesions in paraffin embedded tissues
Abstract: Tyrosinase is a key enzyme in melanin biosynthesis and represents a marker of melanocytic differentiation. We previously generated T311, a murine monoclonal antibody to the tyrosinase recombinant protein. This study was performed to evaluate T311 as a diagnostic immunohistochemical reagent for use on formalin-fixed paraffin-embedded pathological material. We analyzed the specificity of the antibody on a panel of normal and neoplastic tissues, and we assessed its sensitivity in a large number of metastatic and primary malignant melanomas, nevi, three angiomyolipomas, and two vitiligo specimens. T311 revealed intense reactivity on paraffin-embedded material. Immunoreactivity was limited to cells of melanocytic differentiation and no immunostaining was present in unrelated normal tissues and tumors. Eighty- four percent of metastatic malignant melanomas were immunoreactive with T311 and showed predominantly a homogeneous expression pattern. However, in primary melanomas of the desmoplastic/spindle cell type. T311 revealed a poor immunoreactivity. Nevi showed intense staining at the junctional zone, while the dermal component revealed decreasing reactivity towards deeper areas. Only one angiomyolipoma was focally immunoreactive with T311. Vitiligo specimens were immunonegative. We conclude that T311 is a specific and sensitive marker for the detection of melanocytic lesions in formalin-fixed paraffin-embedded tissues and a useful serological reagent for diagnostic pathology.
Keywords: controlled study; human tissue; unclassified drug; diagnostic accuracy; melanoma; melanin; nevus; tumor biopsy; diagnostic value; angiomyolipoma; antibody specificity; monophenol monooxygenase; enzyme assay; vitiligo; tyrosinase; monoclonal antibody t311; enzyme antibody; human; article; t311; melanocyte-associated markers
Journal Title: Pathology Research and Practice
Volume: 196
Issue: 4
ISSN: 0344-0338
Publisher: Elsevier Inc.  
Date Published: 2000-01-01
Start Page: 235
End Page: 242
Language: English
PUBMED: 10782467
PROVIDER: scopus
DOI: 10.1016/S0344-0338(00)80072-2
DOI/URL:
Notes: Export Date: 18 November 2015 -- Source: Scopus
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MSK Authors
  1. Achim Jungbluth
    456 Jungbluth
  2. Klaus J Busam
    688 Busam
  3. Lloyd J Old
    593 Old
  4. Denise Frosina
    123 Frosina
  5. Keren Anne Coplan
    42 Coplan
  6. Kristin A Iversen
    58 Iversen
  7. Yao-Tseng Chen
    83 Chen