Protein expression analysis of melanocyte differentiation antigen TRP-2 Journal Article


Authors: Avogadri, F.; Gnjatic, S.; Tassello, J.; Frosina, D.; Hanson, N.; Laudenbach, M.; Ritter, E.; Merghoub, T.; Busam, K. J.; Jungbluth, A. A.
Article Title: Protein expression analysis of melanocyte differentiation antigen TRP-2
Abstract: Melanocyte differentiation antigens, such as gp100, tyrosinase, and Melan-A and their corresponding antibodies HMB45, T311, and A103, are major diagnostic tools in surgical pathology. Little is known about tyrosinase-related protein 2 (TRP-2, or dopachrome tautomerase/DCT) another melanocyte differentiation antigen, which is an enzymatic component of melanogenesis. We identified a commercial reagent to TRP-2, monoclonal antibody (mAb) C-9 and undertook a comprehensive analysis to assess its specificity and usefulness for surgical pathology. Subsequently, we analyzed panels of normal tissues and tumors. We show that TRP-2 is regularly expressed in melanocytes of the normal skin. In cutaneous nevi, TRP-2 is present in junctional as well as in dermal nevocytes. In malignant tumors, C-9 reactivity is restricted to melanocytic and related lesions and present in 84% and 58% of primary and metastatic melanomas, respectively. Ten primary melanomas of the anorectal mucosa were all positive. Like the other melanocyte differentiation antigens, TRP-2 was absent in 6 desmoplastic melanomas. Also, only 2 of 9 angiomyolipomas were TRP-2 positive. We conclude that mAb C-9 is a valuable reagent for the analysis of TRP-2 expression in archival surgical pathology material. The expression pattern of TRP-2 in melanocytic and related lesions appears to parallel other melanocyte differentiation antigens, although the overall incidence is lower than other antigens, such as Melan-A or gp100. © 2016 Wolters Kluwer Health, Inc.
Keywords: dopachrome tautomerase; melanocyte differentiation antigen; trp-2
Journal Title: American Journal of Dermatopathology
Volume: 38
Issue: 3
ISSN: 0193-1091
Publisher: Lippincott Williams & Wilkins  
Date Published: 2016-03-01
Start Page: 201
End Page: 207
Language: English
DOI: 10.1097/dad.0000000000000362
PROVIDER: scopus
PUBMED: 26894771
PMCID: PMC5844230
DOI/URL:
Notes: Article -- Export Date: 4 April 2016 -- Source: Scopus
Altmetric Score
MSK Authors
  1. Taha Merghoub
    206 Merghoub
  2. Sacha Gnjatic
    111 Gnjatic
  3. Achim Jungbluth
    340 Jungbluth
  4. Erika Ritter
    34 Ritter
  5. Klaus J Busam
    536 Busam
  6. Denise Frosina
    84 Frosina
  7. Nicole C Hanson
    11 Hanson