A103: An anti-Melan-A monoclonal antibody for the detection of malignant melanoma in paraffin-embedded tissues Journal Article
| Authors: | Jungbluth, A. A.; Busam, K. J.; Gerald, W. L.; Stockert, E.; Coplan, K. A.; Iversen, K.; MacGregor, D. P.; Old, L. J.; Chen, Y. T. |
| Article Title: | A103: An anti-Melan-A monoclonal antibody for the detection of malignant melanoma in paraffin-embedded tissues |
| Abstract: | Melan-A is a previously defined, melanocyte differentiation antigen, and an anti-Melan-A murine monoclonal antibody, A103, was recently developed by our group. In this study, we evaluated A103 immunoreactivity on formalin- fixed, paraffin-embedded tissues, exploring the potential of A103 in the diagnosis of metastatic melanoma. Seventy-five metastatic melanomas, 10 primary melanomas, and 10 benign melanocytic nevi were tested. The reactivity of A103 was compared with HMB-4, an anti-gp100 antibody. Results showed that all nevi were A103 positive, and most primary melanomas were A103 and HMB45 positive. Of 75 metastatic melanomas, 61 (81%) were A103 positive, and 56 (75%) were HMB45 positive. Of 19 HMB45-negative lesions, 8 were A103 positive; of 14 A103-negative lesions, 3 were HMB45 positive. Eleven metastatic lesions, as well as 2 of 10 primary melanomas, were dual negative. These negative cases consisted mainly of the spindle cell and desmoplastic variants. Of the positive cases, A103 showed homogeneous staining in a significantly higher proportion of cases than HMB45 (72% versus 52%). In addition, focal staining with less than 5% reactive tumor cells was seen more frequently in HMB45 (12 of 56) than in A103 (5 of 61). These results indicated that A103 can be used as a first-line antibody in the diagnosis of metastatic melanoma. Our results also showed that A103 reacted with angiomyolipoma, which is known to be HMB45 positive. Of normal tissues, unexpected A 103 reactivity was observed in the adrenal cortex, granulosa and theca cells of the ovary, and Leydig cells of the testis. This A103 immunoreactivity in benign and neoplastic tissues of nonmelanocytic origin, the basis of which is unclear, could also be of potential diagnostic value. |
| Keywords: | immunohistochemistry; controlled study; human tissue; unclassified drug; human cell; major clinical study; cancer diagnosis; melanoma; metastasis; nevus; skin neoplasms; melanocyte; tumor differentiation; neoplasm proteins; leydig cell; monoclonal antibody; antibodies, monoclonal; antigens, neoplasm; tissue distribution; melanocytic nevus; diagnostic value; angiomyolipoma; spindle cell; hybridoma; formaldehyde; paraffin; differentiation antigen; paraffin embedding; monoclonal antibody hmb 45; granulosa cell; embedding; melan-a; adrenal cortex; monoclonal antibody a103; mart-1; humans; human; male; female; article; hmb45; theca cell |
| Journal Title: | American Journal of Surgical Pathology |
| Volume: | 22 |
| Issue: | 5 |
| ISSN: | 0147-5185 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 1998-05-01 |
| Start Page: | 595 |
| End Page: | 602 |
| Language: | English |
| DOI: | 10.1097/00000478-199805000-00011 |
| PUBMED: | 9591730 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work