Immunohistochemical analysis of novel monoclonal antibody PNL2 and comparison with other melanocyte differentiation markers Journal Article
| Authors: | Busam, K. J.; Kucukgol, D.; Sato, E.; Frosina, D.; Teruya-Feldstein, J.; Jungbluth, A. A. |
| Article Title: | Immunohistochemical analysis of novel monoclonal antibody PNL2 and comparison with other melanocyte differentiation markers |
| Abstract: | PNL2 is a novel monoclonal antibody, which has recently been introduced as an immunohistochemical reagent to stain melanocyte and tumors derived thereof. In the present study, we analyzed the immunoreactivity of this mAb in various normal tissues, melanocytic nevi, primary and metastatic melanoma, nonmelanocytic tumors, including histologic mimickers of melanoma as well as angiomyolipoma, and multiple cell lines derived from different tumors types. We used several tissue microarray panels as well as selected conventional sections from tissue blocks. For metastatic melanoma, immunoreactivity for PNL2 was compared with A103 (Melan-A/MART-1), T311 (tyrosinase), HMB45 (gp100), and D5 (MITF). Positive staining with PNL2 was found in normal melanocytes and neutrophils, but no other normal cell type. Among melanocytic lesions, both benign nevi as well as primary malignant melanomas, especially epithelioid variants thereof, were commonly immunopositive. Only 1 of 13 desmoplastic melanomas reacted with PNL2. PNL2 showed high sensitivity for metastatic melanoma (87%). In comparison, 82% of metastatic melanomas were positive for A103, 76% for HMB45, 92% for T311, and 84% for D5. The combined use of all five reagents minimized the number of immunonegative cases. None of the selected non-melanocytic tumors (carcinomas or soft tissue neoplasms) was positive for PNL2 in this series except for angiomyolipomas and chronic myeloid leukemias and 1 single case of a malignant peripheral nerve sheath tumor with heterologous differentiation (malignant Triton tumor). Despite its reactivity with neutrophils, PNL2 appears to be a valuable supplementary reagent for the diagnosis of melanocytic tumors. Copyright © 2005 by Lippincott Williams & Wilkins. |
| Keywords: | immunohistochemistry; controlled study; unclassified drug; human cell; comparative study; diagnostic accuracy; sensitivity and specificity; biological markers; glycoprotein gp 100; melanoma; metastasis; nevus; skin neoplasms; melanocyte; melanocytes; cell differentiation; chronic myeloid leukemia; immunoreactivity; monoclonal antibody; antibodies, monoclonal; melanocytic nevus; angiomyolipoma; tissue array analysis; melan a; monophenol monooxygenase; tissue microarray; nerve sheath tumor; melanocyte differentiation; pnl2; monoclonal antibody a103; monoclonal antibody d5; monoclonal antibody hmb45; monoclonal antibody pnl2; monoclonal antibody t311 |
| Journal Title: | American Journal of Surgical Pathology |
| Volume: | 29 |
| Issue: | 3 |
| ISSN: | 0147-5185 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 2005-03-01 |
| Start Page: | 400 |
| End Page: | 406 |
| Language: | English |
| DOI: | 10.1097/01.pas.0000152137.81771.5b |
| PUBMED: | 15725810 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 28" - "Export Date: 24 October 2012" - "CODEN: AJSPD" - "Source: Scopus" |
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