The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G2 phase Journal Article
| Authors: | Izumi, M.; Yanagi, K. I.; Mizuno, T.; Yokoi, M.; Kawasaki, Y.; Moon, K. Y.; Hurwitz, J.; Yatagai, F.; Hanaoka, F. |
| Article Title: | The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G2 phase |
| Abstract: | Mcm10 (Dna43), first identified in Saccharomyces cerevisiae, is an essential protein which functions in the initiation of DNA synthesis. Mcm 10 is a nuclear protein that is localized to replication origins and mediates the interaction of the Mcm2-7 complex with replication origins. We identified and cloned a human cDNA whose product was structurally homologous to the yeast Mcm10 protein. Human Mcm 10 (HsMcm10) is a 98-kDa protein of 874 amino acids which shows 23 and 21% overall similarity to Schizosaccharomyces pombe Cdc23 and S. cerevisiae Mcm 10, respectively. The messenger RNA level of HsMcm10 increased at the G 1/S-boundary when quiescent human NB1-RGB cells were induced to proliferate as is the case of many replication factors. HsMcm10 associated with nuclease-resistant nuclear structures throughout S phase and dissociated from it in G 2 phase. HsMcm10 associated with human Orc2 protein when over-expressed in COS-1 cells. HsMcm10 also interacted with Orc2, Mcm2 and Mcm6 proteins in the yeast two-hybrid system. These results suggest that HsMcm10 may function in DNA replication through the interaction with Orc and Mcm2-7 complexes. |
| Keywords: | controlled study; unclassified drug; dna-binding proteins; nonhuman; dna replication; dna synthesis; protein conformation; protein localization; cell proliferation; animal cell; animals; cell cycle proteins; cell cycle; cell cycle s phase; gene expression; nuclear protein; protein protein interaction; cell protein; cell line; protein binding; hela cells; cos cells; animalia; molecular cloning; amino acid sequence; molecular sequence data; sequence homology, amino acid; messenger rna; saccharomyces cerevisiae; rna, messenger; sequence alignment; eukaryota; nucleotide sequence; cell strain cos1; cellular distribution; species difference; nuclease; plasmids; amino acid; cell cycle g2 phase; cell nucleus; saccharomyces cerevisiae proteins; replication factor c; dna replication origin; sequence homology; protein determination; cell cycle g1 phase; fungal protein; complementary dna; schizosaccharomyces pombe proteins; g2 phase; dna, complementary; sequence analysis, dna; schizosaccharomyces pombe; two-hybrid system techniques; minichromosome maintenance protein 2; minichromosome maintenance protein 3; fungal dna; deoxyribonucleases; origin recognition complex; protein mcm10; growth stimulation; dna, recombinant; precipitin tests; humans; human; priority journal; article; protein mcm4; protein mcm5; protein mcm6; protein mcm7; protein orc2 |
| Journal Title: | Nucleic Acids Research |
| Volume: | 28 |
| Issue: | 23 |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Date Published: | 2000-12-01 |
| Start Page: | 4769 |
| End Page: | 4777 |
| Language: | English |
| PUBMED: | 11095689 |
| PROVIDER: | scopus |
| PMCID: | PMC115166 |
| DOI: | 10.1093/nar/28.23.4769 |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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