Yeast ORC sumoylation status fine-tunes origin licensing Journal Article
| Authors: | Regan-Mochrie, G.; Hoggard, T.; Bhagwat, N.; Lynch, G.; Hunter, N.; Remus, D.; Fox, C. A.; Zhao, X. |
| Article Title: | Yeast ORC sumoylation status fine-tunes origin licensing |
| Abstract: | Sumoylation is emerging as a posttranslation modification important for regulating chromosome duplication and stability. The origin recognition complex (ORC) that directs DNA replication initiation by loading the MCM replicative helicase onto origins is sumoylated in both yeast and human cells. However, the biological consequences of ORC sumoylation are unclear. Here we report the effects of hypersumoylation and hyposumoylation of yeast ORC on ORC activity and origin function using multiple approaches. ORC hypersumoylation preferentially reduced the function of a subset of early origins, while Orc2 hyposumoylation had an opposing effect. Mechanistically, ORC hypersumoylation reduced MCM loading in vitro and diminished MCM chromatin association in vivo. Either hypersumoylation or hyposumoylation of ORC resulted in genome instability and the dependence of yeast on other genome maintenance factors, providing evidence that appropriate ORC sumoylation levels are important for cell fitness. Thus, yeast ORC sumoylation status must be properly controlled to achieve optimal origin function across the genome and genome stability. © 2022 Regan-Mochrie et al. |
| Keywords: | controlled study; nonhuman; in vivo study; in vitro study; genomic instability; yeast; sumoylation; yeast cell; fungus growth; fungal dna; fungal genome; origin recognition complex; mcm; article; replication initiation; orc; replication regulation |
| Journal Title: | Genes and Development |
| Volume: | 36 |
| Issue: | 13-14 |
| ISSN: | 0890-9369 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Date Published: | 2022-07-01 |
| Start Page: | 807 |
| End Page: | 821 |
| Language: | English |
| DOI: | 10.1101/gad.349610.122 |
| PUBMED: | 35926881 |
| PROVIDER: | scopus |
| PMCID: | PMC9480853 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 November 2022 -- Source: Scopus |
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