Post-licensing specification of eukaryotic replication origins by facilitated Mcm2-7 sliding along DNA Journal Article
| Authors: | Gros, J.; Kumar, C.; Lynch, G.; Yadav, T.; Whitehouse, I.; Remus, D. |
| Article Title: | Post-licensing specification of eukaryotic replication origins by facilitated Mcm2-7 sliding along DNA |
| Abstract: | Eukaryotic genomes are replicated from many origin sites that are licensed by the loading of the replicative DNA helicase, Mcm2-7. How eukaryotic origin positions are specified remains elusive. Here we show that, contrary to the bacterial paradigm, eukaryotic replication origins are not irrevocably defined by selection of the helicase loading site, but can shift in position after helicase loading. Using purified proteins we show that DNA translocases, including RNA polymerase, can push budding yeast Mcm2-7 double hexamers along DNA. Displaced Mcm2-7 double hexamers support DNA replication initiation distal to the loading site in vitro. Similarly, in yeast cells that are defective for transcription termination, collisions with RNA polymerase induce a redistribution of Mcm2-7 complexes along the chromosomes, resulting in a corresponding shift in DNA replication initiation sites. These results reveal a eukaryotic origin specification mechanism that departs from the classical replicon model, helping eukaryotic cells to negotiate transcription-replication conflict. Eukaryotic replication origins are licensed by the loading of the replicative helicase, Mcm2-7, in inactive double hexameric form around DNA. Gros et al. report that RNA polymerases and other DNA translocases can push Mcm2-7 double hexamers along DNA prior to Mcm2-7 activation, thereby influencing the position of replication origin sites. © 2015 Elsevier Inc. |
| Keywords: | nonhuman; dna replication; protein localization; complex formation; protein purification; dna replication origin; dna helicase; eukaryote; rna polymerase; transcription termination; replicon; minichromosome maintenance protein 2; minichromosome maintenance protein 7; article |
| Journal Title: | Molecular Cell |
| Volume: | 60 |
| Issue: | 5 |
| ISSN: | 1097-2765 |
| Publisher: | Cell Press |
| Date Published: | 2015-12-03 |
| Start Page: | 797 |
| End Page: | 807 |
| Language: | English |
| DOI: | 10.1016/j.molcel.2015.10.022 |
| PROVIDER: | scopus |
| PMCID: | PMC4680849 |
| PUBMED: | 26656162 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 3 February 2016 -- Source: Scopus |
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