Eukaryotic replication origins: Strength in flexibility Journal Article
| Authors: | Kumar, C.; Remus, D. |
| Article Title: | Eukaryotic replication origins: Strength in flexibility |
| Abstract: | ABSTRACT: The eukaryotic replicative DNA helicase, Mcm2-7, is loaded in inactive form as a double hexameric complex around double-stranded DNA. To ensure that replication origins fire no more than once per S phase, activation of the Mcm2-7 helicase is temporally separated from Mcm2-7 loading in the cell cycle. This 2-step mechanism requires that inactive Mcm2-7 complexes be maintained for variable periods of time in a topologically bound state on chromatin, which may create a steric obstacle to other DNA transactions. We have recently found in the budding yeast, Saccharomyces cerevisiae, that Mcm2-7 double hexamers can respond to collisions with transcription complexes by sliding along the DNA template. Importantly, Mcm2-7 double hexamers remain functional after displacement along DNA and support replication initiation from sites distal to the origin. These results reveal a novel mechanism to specify eukaryotic replication origin sites and to maintain replication origin competence without the need for Mcm2-7 reloading. © 2016 The Author(s). Published with license by Taylor & Francis Group, LLC. |
| Keywords: | dna replication; chromosomes; replication origin; mcm2-7; orc |
| Journal Title: | Nucleus |
| Volume: | 7 |
| Issue: | 3 |
| ISSN: | 1949-1034 |
| Publisher: | Taylor & Francis Group |
| Date Published: | 2016-01-01 |
| Start Page: | 292 |
| End Page: | 300 |
| Language: | English |
| DOI: | 10.1080/19491034.2016.1187353 |
| PROVIDER: | scopus |
| PUBMED: | 27416360 |
| PMCID: | PMC4991242 |
| DOI/URL: | |
| Notes: | Note -- Export Date: 2 August 2016 -- Source: Scopus |
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