Rat protein kinase C zeta gene contains alternative promoters for generation of dual transcripts with 5′-end heterogeneity Journal Article
| Authors: | Marshall, B. S.; Price, G.; Powell, C T. |
| Article Title: | Rat protein kinase C zeta gene contains alternative promoters for generation of dual transcripts with 5′-end heterogeneity |
| Abstract: | Protein kinase C (PKC) ζ is a phospholipid-dependent serine/threonine kinase that appears to perform important cell signaling functions. Two forms of PKC ζ RNA, with different 5′ ends, have been reported. The major form (ζ) is expressed in most, if not all tissues, while the minor form (ζ′), which encodes the catalytic domain of the enzyme without most of its regulatory domain, is predominant in normal brain and certain rat prostate tumors. We report here tile structure of the 5′ end of the rat PKC ζ gene, demonstrating that both forms of RNA can be transcribed from the same gene through the use of alternative promoters and splicing. In luciferase reporter constructs, progressive deletions of the PKC ζ and ζ′ 5′ flanking sequences yielded activities that were higher in the cell lines expressing endogenous PKC ζ and ζ′ RNAs, respectively. Also, multiple PCRs across different introns of the PKC ζ gene indicate that recent duplication of the gene or the existence of a closely related pseudogene in the rat genome are unlikely. |
| Keywords: | unclassified drug; promoter region; gene deletion; exons; sequence deletion; nonhuman; polymerase chain reaction; animal cell; animals; luciferase; intron; introns; animalia; rna; gene expression regulation; cloning, molecular; molecular sequence data; nucleotide sequence; substrate specificity; rat; alternative rna splicing; reporter gene; gene duplication; base sequence; dna flanking region; protein kinase c; genes, reporter; rats; response elements; rodentia; dna primers; rats, sprague-dawley; rna structure; 5' untranslated regions; rna splice sites; protein kinase c zeta; pseudogene; pseudogenes; enhancer elements (genetics); promoter regions (genetics); priority journal; article; genes, duplicate; nuclease protection assays |
| Journal Title: | DNA and Cell Biology |
| Volume: | 19 |
| Issue: | 12 |
| ISSN: | 1044-5498 |
| Publisher: | Mary Ann Liebert, Inc |
| Date Published: | 2000-12-01 |
| Start Page: | 707 |
| End Page: | 719 |
| Language: | English |
| DOI: | 10.1089/104454900750058071 |
| PUBMED: | 11177569 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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