Differential expression of protein kinase C isozyme messenger RNAs in Dunning R-3327 rat prostatic tumors Journal Article
| Authors: | Powell, C. T.; Fair, W. R.; Heston, W. D. W. |
| Article Title: | Differential expression of protein kinase C isozyme messenger RNAs in Dunning R-3327 rat prostatic tumors |
| Abstract: | Adenocarcinoma of the prostate is the second leading cause of cancer deaths in men. The protein kinase C (PKC) family of signal transducing kinases has been implicated in neoplastic transformation and progression in other tissues, and some evidence suggests roles for PKC in prostate growth and neoplasia. We have detected expression of eight specific PKC isozyme mRNAs (α, β, γ, δ, ε, θ, and ζ) in normal rat whole prostate and found some of these to be differentially expressed in certain Dunning R-3327 rat prostatic adenocarcinoma sublines. PKC ζ mRNA was detected in normal prostate and Dunning H tumor, whereas an alternatively spliced form of PKC ζ RNA was found in Dunning G tumor and normal brain. Both forms of PKC ζ RNA were markedly reduced in the androgen insensitive, highly metastatic Dunning AT-3, MAT-Lu, and MAT-LyLu tumors. We have cloned and report the sequence of the novel portion of the alternatively spliced form of PKC ζ RNA, which is polyadenylated and present in cytoplasm. |
| Keywords: | signal transduction; controlled study; nonhuman; polymerase chain reaction; adenocarcinoma; animal cell; animal; animal tissue; cell division; gene expression; cell line; animal model; tumor cells, cultured; prostatic neoplasms; dna; molecular sequence data; kinetics; brain; messenger rna; rna, messenger; rat; base sequence; protein kinase c; rats; prostate adenocarcinoma; receptors, androgen; isoenzymes; rna sequence; dna, complementary; cloning; rna probes; isoenzyme; male; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Cell Growth & Differentiation |
| Volume: | 5 |
| Issue: | 2 |
| ISSN: | 1044-9523 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1994-02-01 |
| Start Page: | 143 |
| End Page: | 149 |
| Language: | English |
| PROVIDER: | scopus |
| PUBMED: | 8180127 |
| DOI/URL: | |
| Notes: | Export Date: 14 January 2019 -- Article -- Source: Scopus |
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