Overexpression of protein kinase C-zeta (PKC-ζ) inhibits invasive and metastatic abilities of Dunning R-3327 MAT-LyLu rat prostate cancer cells Journal Article
| Authors: | Powell, C. T.; Gschwend, J. E.; Fair, W. R.; Brittis, N. J.; Stec, D.; Huryk, R. |
| Article Title: | Overexpression of protein kinase C-zeta (PKC-ζ) inhibits invasive and metastatic abilities of Dunning R-3327 MAT-LyLu rat prostate cancer cells |
| Abstract: | Previously, we reported that protein kinase C (PKC)-ζ mRNA levels are reduced markedly in metastatic Dunning R-3327 rat prostate tumors relative to the nonmetastatic Dunning H tumor and normal rat prostate (C. T. Powell et al., Cell Growth and Differ., 5: 143-149, 1994). To examine the effect of PKC-ζ on metastatic and invasive abilities of an aggressive Dunning R-3327 cell line, we generated stably transfected clones of MAT-LyLu cells that overexpress active PKC-ζ. PKC-ζ-overexpressing MAT-LyLu cells exhibited tumorigenicity and growth rates in syngeneic rats similar to those of MAT- LyLu cells transfected with vector alone or untransfected MAT-LyLu. However, nine independent clones of PKC-ζ expressing cells exhibited an average 2- fold lower tendency to metastasize to lungs relative to vector-transfected MAT-LyLu cell clones, with about 2-fold and 4.5-fold fewer metastases per rat in two separate protocols. In addition, the ability of four PKC-ζ- overexpressing MAT-LyLu clones to invade through Matrigel in a Boyden chamber assay was reduced an average of 12-fold relative to three vector-transfected clones. These results indicate that increased PKC-ζ expression can substantially suppress invasion and metastasis by an aggressive rat prostate tumor. |
| Keywords: | controlled study; cancer growth; nonhuman; animals; animal tissue; metastasis; gene expression; cell line; animal experiment; animal model; cancer cell culture; tumor cells, cultured; transfection; genetic vectors; prostate cancer; cancer invasion; prostatic neoplasms; molecular cloning; cloning, molecular; prostate; rna, messenger; recombinant proteins; rat; carcinogenicity; neoplasm metastasis; collagen; protein kinase c; rats; neoplasm invasiveness; drug combinations; laminin; transplantation, isogeneic; proteoglycans; avian sarcoma viruses; male; priority journal; article |
| Journal Title: | Cancer Research |
| Volume: | 56 |
| Issue: | 18 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1996-09-15 |
| Start Page: | 4137 |
| End Page: | 4141 |
| Language: | English |
| PUBMED: | 8797581 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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