Overexpression of protein kinase C βI in a murine keratinocyte cell line produces effects on cellular growth, morphology and differentiation Journal Article


Authors: O'Driscoll, K. R.; Madden, P. V.; Christiansen, K. M.; Viage, A.; Slaga, T. J.; Fabbro, D.; Powell, C. T.; Weinstein, I. B.
Article Title: Overexpression of protein kinase C βI in a murine keratinocyte cell line produces effects on cellular growth, morphology and differentiation
Abstract: The present study demonstrates that the murine keratinocyte cell line 3PC expresses the Ca2+-insensitive isoforms of protein kinase C (PKC) δ, ε{lunate}, ζ and (at both the mRNA and protein levels), but does not express the Ca2+-sensitive PKC isoforms α, β or γ. Recombinant retroviral gene transduction was used to develop derivatives of this cell line that stably express high levels of 1 PKCβI-related transcripts and proteins, and have increased levels of Ca2+-stimulated PKC enzyme activity. Functional overexpression of the PKCβI isoform in 3PC cells enhances both 12-O-tetradecanoyl phorbol-13-acetate-induced growth inhibition, and Ca2+-induced morphologic differentiation. © 1994.
Keywords: controlled study; nonhuman; animal cell; mouse; animal; mice; cell division; cell structure; gene expression; cell growth; cell line; calcium; cell differentiation; enzyme activity; keratinocyte; genetic transduction; animalia; rna, messenger; murinae; protein kinase c; tumor promotion; keratinocytes; isoenzymes; growth inhibition; phorbol 13 acetate 12 myristate; tetradecanoylphorbol acetate; multistage carcinogenesis; isoenzyme; calcium ion; epidermal differentiation; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; 12-o-tetradecanoyl phorbol-13-acetate; murine keratinocytes; protein kinase c isoforms
Journal Title: Cancer Letters
Volume: 83
Issue: 1-2
ISSN: 0304-3835
Publisher: Elsevier Ireland Ltd.  
Date Published: 1994-08-15
Start Page: 249
End Page: 259
Language: English
DOI: 10.1016/0304-3835(94)90327-1
PROVIDER: scopus
PUBMED: 8062223
DOI/URL:
Notes: Export Date: 14 January 2019 -- Article -- Source: Scopus
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  1. C Thomas Powell
    36 Powell