Overexpression of protein kinase C βI in a murine keratinocyte cell line produces effects on cellular growth, morphology and differentiation Journal Article
| Authors: | O'Driscoll, K. R.; Madden, P. V.; Christiansen, K. M.; Viage, A.; Slaga, T. J.; Fabbro, D.; Powell, C. T.; Weinstein, I. B. |
| Article Title: | Overexpression of protein kinase C βI in a murine keratinocyte cell line produces effects on cellular growth, morphology and differentiation |
| Abstract: | The present study demonstrates that the murine keratinocyte cell line 3PC expresses the Ca2+-insensitive isoforms of protein kinase C (PKC) δ, ε{lunate}, ζ and (at both the mRNA and protein levels), but does not express the Ca2+-sensitive PKC isoforms α, β or γ. Recombinant retroviral gene transduction was used to develop derivatives of this cell line that stably express high levels of 1 PKCβI-related transcripts and proteins, and have increased levels of Ca2+-stimulated PKC enzyme activity. Functional overexpression of the PKCβI isoform in 3PC cells enhances both 12-O-tetradecanoyl phorbol-13-acetate-induced growth inhibition, and Ca2+-induced morphologic differentiation. © 1994. |
| Keywords: | controlled study; nonhuman; animal cell; mouse; animal; mice; cell division; cell structure; gene expression; cell growth; cell line; calcium; cell differentiation; enzyme activity; keratinocyte; genetic transduction; animalia; rna, messenger; murinae; protein kinase c; tumor promotion; keratinocytes; isoenzymes; growth inhibition; phorbol 13 acetate 12 myristate; tetradecanoylphorbol acetate; multistage carcinogenesis; isoenzyme; calcium ion; epidermal differentiation; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; 12-o-tetradecanoyl phorbol-13-acetate; murine keratinocytes; protein kinase c isoforms |
| Journal Title: | Cancer Letters |
| Volume: | 83 |
| Issue: | 1-2 |
| ISSN: | 0304-3835 |
| Publisher: | Elsevier Ireland Ltd. |
| Date Published: | 1994-08-15 |
| Start Page: | 249 |
| End Page: | 259 |
| Language: | English |
| DOI: | 10.1016/0304-3835(94)90327-1 |
| PROVIDER: | scopus |
| PUBMED: | 8062223 |
| DOI/URL: | |
| Notes: | Export Date: 14 January 2019 -- Article -- Source: Scopus |
