Introduction of the β isozyme of protein kinase C accelerates induced differentiation of murine erythroleukemia cells Journal Article

   


Authors: Melloni, E.; Pontremoli, S.; Sparatore, B.; Patrone, M.; Grossi, F.; Marks, P. A.; Rifkind, R. A.
Article Title: Introduction of the β isozyme of protein kinase C accelerates induced differentiation of murine erythroleukemia cells
Abstract: Induction of differentiation in murine erythroleukemia cells (MELCs) involves a protein kinase C (PKC)-mediated step. Vincristine-resistant cells respond more rapidly to hybrid polar/apolar inducers than the parental cells. These vineristine-resistant MELCs contain elevated levels of the β isozyme of PKC (PKC-β). Exogenous homologous murine PKC-β, incorporated into permeabilized MELCs, accelerates induced differentiation. Neither rat PKC-β, nor mouse PKC-α, rat PKC-α, incorporated into permeabilized MELCs, is effective in altering the kinetics of induced differentiation. This provides direct evidence for a rate-limiting role for this PKC isozyme during N′,N′-hexamethylenebisacetamide-mediated induced differentiation of a transformed cell.
Keywords: controlled study; unclassified drug; nonhuman; animal cell; mouse; animal; mice; cell division; cell line; animal experiment; hemoglobin; vincristine; cell differentiation; drug resistance; tumor cells, cultured; animalia; kinetics; cell culture; brain; murinae; immunoblotting; protein kinase c; rats; cell density; enzyme purification; isoenzymes; protein kinase c alpha; induction; isoenzyme; protein kinase c beta; leukemia, erythroblastic, acute; commitment; hexamethylenebisacetamide; acetamides; priority journal; article; leukemia, experimental; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; erythroleukemia cell; beta isoenzyme
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 87
Issue: 12
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1990-06-01
Start Page: 4417
End Page: 4420
Language: English
DOI: 10.1073/pnas.87.12.4417
PUBMED: 2352925
PROVIDER: scopus
PMCID: PMC54125
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025358366&doi=10.1073%2fpnas.87.12.4417&partnerID=40&md5=d0016341f6c1b8f3427ca6114e883d35
Notes: Article -- Export Date: 27 January 2020 -- Source: Scopus
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MSK Authors
  1. Paul Marks
    186 Marks
  2. Richard Rifkind
    118 Rifkind
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