Protein kinase C isozymes ε and α in murine erythroleukemia cells Journal Article
| Authors: | Powell, C. T.; Leng, L.; Dong, L.; Kiyokawa, H.; Busquets, X.; O'Driscoll, K.; Marks, P. A.; Rifkind, R. A. |
| Article Title: | Protein kinase C isozymes ε and α in murine erythroleukemia cells |
| Abstract: | Protein kinase C (PKC) has a role in signal transduction during hexamethylene bisacetamide (HMBA)-induced differentiation of murine erythroleukemia cells (MELC). Separation of MELC PKC isozymes by hydroxylapatite chromatography yields a major peak (III) and a minor peak (II) of PKC activity, previously reported to contain the PKC α and β isozymes, respectively. In the present study, we confirm that peak III activity is PKCα but show that peak II contains PKCε and little or no PKCβ. Immunoblot analysis with isozyme-specific anti-α and anti-ε PKC antibodies detected PKCα in peak III and PKCε in peak II. Peak III activity was markedly enhanced (up to 20-fold) by phosphatidylserine, diolein, and Ca2+, whereas addition of these cofactors to the reaction mixture stimulated peak II activity only 2- to 4-fold. RNase protection analysis of MELC RNA showed that PKCα and PKCε RNAs were in a ratio of ≃2:1, but PKCβ RNA was barely detectable. Taken together, these data indicate that MELC contain PKCα and PKCε but little or no PKCβ. |
| Keywords: | signal transduction; nonhuman; mouse; murinae; protein kinase; isoenzyme; priority journal; article; erythroleukemia cell |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 89 |
| Issue: | 1 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1992-01-01 |
| Start Page: | 147 |
| End Page: | 151 |
| Language: | English |
| DOI: | 10.1073/pnas.89.1.147 |
| PUBMED: | 1729681 |
| PROVIDER: | scopus |
| PMCID: | PMC48193 |
| DOI/URL: | |
| Notes: | Source: Scopus |
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