RecF and RecR play critical roles in the homologous recombination and single-strand annealing pathways of mycobacteria Journal Article
| Authors: | Gupta, R.; Shuman, S.; Glickman, M. S. |
| Article Title: | RecF and RecR play critical roles in the homologous recombination and single-strand annealing pathways of mycobacteria |
| Abstract: | Mycobacteria encode three DNA double-strand break repair pathways: (i) RecA-dependent homologous recombination (HR), (ii) Ku-dependent nonhomologous end joining (NHEJ), and (iii) RecBCD-dependent single-strand annealing (SSA). Mycobacterial HR has two presynaptic pathway options that rely on the helicase-nuclease AdnAB and the strand annealing protein RecO, respectively. Ablation of adnAB or recO individually causes partial impairment of HR, but loss of adnAB and recO in combination abolishes HR. RecO, which can accelerate annealing of single-stranded DNA in vitro, also participates in the SSA pathway. The functions of RecF and RecR, which, in other model bacteria, function in concert with RecO as mediators of RecA loading, have not been examined in mycobacteria. Here, we present a genetic analysis of recF and recR in mycobacterial recombination. We find that RecF, like RecO, participates in the AdnAB-independent arm of the HR pathway and in SSA. In contrast, RecR is required for all HR in mycobacteria and for SSA. The essentiality of RecR as an agent of HR is yet another distinctive feature of mycobacterial DNA repair. © 2015, American Society for Microbiology. |
| Keywords: | nonhuman; genetic analysis; homologous recombination; chemical reaction; bacterial gene; mycobacterium smegmatis; single strand annealing; priority journal; article; recr protein; recf protein; recf gene; recr gene |
| Journal Title: | Journal of Bacteriology |
| Volume: | 197 |
| Issue: | 19 |
| ISSN: | 0021-9193 |
| Publisher: | American Society for Microbiology |
| Date Published: | 2015-10-01 |
| Start Page: | 3121 |
| End Page: | 3132 |
| Language: | English |
| DOI: | 10.1128/jb.00290-15 |
| PROVIDER: | scopus |
| PUBMED: | 26195593 |
| PMCID: | PMC4560283 |
| DOI/URL: | |
| Notes: | Export Date: 2 October 2015 -- Source: Scopus |
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