RecO protein initiates DNA recombination and strand annealing through two alternative DNA binding mechanisms Journal Article
| Authors: | Ryzhikov, M.; Gupta, R.; Glickman, M.; Korolev, S. |
| Article Title: | RecO protein initiates DNA recombination and strand annealing through two alternative DNA binding mechanisms |
| Abstract: | Recombination mediator proteins (RMPs) are important for genome stability in all organisms. Several RMPs support two alternative reactions: initiation of homologous recombination and DNA annealing. We examined mechanisms of RMPs in both reactions with Mycobacterium smegmatis RecO (MsRecO) and demonstrated that MsRecO interacts with ssDNA by two distinct mechanisms. Zincstimulates MsRecO binding to ssDNAduring annealing, whereas the recombination function is zinc-independent and is regulated by interaction with MsRecR. Thus, different structural motifs or conformations of MsRecO are responsible for interaction with ssDNA during annealing and recombination. Neither annealing nor recombinase loading depends on MsRecO interaction with the conserved C-terminal tail of single-stranded (ss) DNA-binding protein (SSB), which is known to bind Escherichia coli RecO. However, similarly to E. coli proteins, MsRecO and MsRecOR do not dismiss SSB from ssDNA, suggesting that RMPs form a complex with SSB-ssDNA even in the absence of binding to the major protein interaction motif. We propose that alternative conformations of such complexes define the mechanism by which RMPs initiate the repair of stalled replication and support two different functions during recombinational repair of DNA breaks. |
| Keywords: | controlled study; nonhuman; protein conformation; protein function; protein analysis; dna recombination; homologous recombination; complex formation; carboxy terminal sequence; protein dna binding; escherichia coli; adenosine triphosphate; single stranded dna; zinc; dna binding; protein dna interaction; mycobacterium smegmatis; protein hydrolysis; zinc finger motif; reca protein; single stranded dna binding protein; recombination repair; reco protein; article; strand annealing; recr protein |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 289 |
| Issue: | 42 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2014-10-17 |
| Start Page: | 28846 |
| End Page: | 28855 |
| Language: | English |
| DOI: | 10.1074/jbc.M114.585117 |
| PROVIDER: | scopus |
| PMCID: | PMC4200245 |
| PUBMED: | 25170075 |
| DOI/URL: | |
| Notes: | Export Date: 1 December 2014 -- Source: Scopus |
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