Structure and conformational change of a replication protein A heterotrimer bound to ssDNA Journal Article
| Authors: | Fan, J.; Pavletich, N. P. |
| Article Title: | Structure and conformational change of a replication protein A heterotrimer bound to ssDNA |
| Abstract: | Replication protein A (RPA) is the main eukaryotic ssDNA-binding protein with essential roles in DNA replication, recombination, and repair. RPA maintains the DNA as single-stranded and also interacts with other DNA-processing proteins, coordinating their assembly and disassembly on DNA. RPA binds to ssDNA in two conformational states with opposing affinities for DNA and proteins. The RPA-protein interactions are compatible with a low DNA affinity state that involves DNA-binding domain A (DBD-A) and DBD-B but not with the high DNA affinity state that additionally engages DBD-C and DBD-D. The structure of the high-affinity RPA-ssDNA complex reported here shows a compact quaternary structure held together by a four-way interface between DBD-B, DBD-C, the intervening linker (BC linker), and ssDNA. The BC linker binds into the DNAbinding groove of DBD-B, mimicking DNA. The associated conformational change and partial occlusion of the DBD-A-DBA-B protein-protein interaction site establish a mechanism for the allosteric coupling of RPA-DNA and RPA-protein interactions. © 2012 by Cold Spring Harbor Laboratory Press. |
| Keywords: | controlled study; protein expression; nonhuman; binding affinity; dna replication; protein conformation; animal cell; dna recombination; dna repair; protein protein interaction; cell line; protein binding; models, molecular; protein structure, tertiary; replication factor a; single stranded dna; dna, single-stranded; nucleic acid conformation; protein structure; dna binding; replication protein a; protein dna interaction; allosterism; cross coupling reaction; fungal proteins; insect cell; ustilago; rpa; ssdna binding |
| Journal Title: | Genes and Development |
| Volume: | 26 |
| Issue: | 20 |
| ISSN: | 0890-9369 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Date Published: | 2012-10-15 |
| Start Page: | 2337 |
| End Page: | 2347 |
| Language: | English |
| DOI: | 10.1101/gad.194787.112 |
| PROVIDER: | scopus |
| PMCID: | PMC3475805 |
| PUBMED: | 23070815 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 2 November 2012" - "CODEN: GEDEE" - "Source: Scopus" |
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