Human RPA (hSSB) interacts with EBNA1, the latent origin binding protein of Epstein-Barr virus Journal Article

   


Authors: Zhang, D.; Frappier, L.; Gibbs, E.; Hurwitz, J.; O'Donnell, M.
Article Title: Human RPA (hSSB) interacts with EBNA1, the latent origin binding protein of Epstein-Barr virus
Abstract: RPA is the replicative single-strand DNA (ssDNA) binding protein of eukaryotic chromosomes. This report shows that human RPA interacts with EBNA1, the latent origin binding protein of Epstein-Barr virus (EBV). RPA binds to EBNA1 both in solution, and when EBNA1 is bound to the EBV origin. RPA is a heterotrimer, and the main contact with EBNA1 is formed through the 70 kDa subunit of RPA, the subunit which binds to ssDNA. We propose that this interaction between RPA and EBNA1 is an early step in activation of the latent origin of EBV.
Keywords: dna-binding proteins; nonhuman; chromosome; protein protein interaction; eukaryota; escherichia coli; peptide fragments; recombinant proteins; dna, viral; dimerization; protein subunit; virus latency; epstein barr virus; replication protein a; herpesvirus 4, human; virus protein; human herpesvirus 4; single stranded dna binding protein; humans; human; priority journal; article; epstein-barr virus nuclear antigens
Journal Title: Nucleic Acids Research
Volume: 26
Issue: 2
ISSN: 0305-1048
Publisher: Oxford University Press  
Date Published: 1998-01-01
Start Page: 631
End Page: 637
Language: English
DOI: 10.1093/nar/26.2.631
PUBMED: 9421526
PROVIDER: scopus
PMCID: PMC147273
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032518150&doi=10.1093%2fnar%2f26.2.631&partnerID=40&md5=94ba695da8b1488fa3ecf2517c7db6fb
Notes: Article -- Export Date: 12 December 2016 -- Source: Scopus
Altmetric
What is Altmetric?
Citation Impact
What is Dimensions Citation Badge?
BMJ Impact Analytics
MSK Authors
  1. Jerard Hurwitz
    206 Hurwitz
  2. Emma Gibbs
    14 Gibbs
Related MSK Work