A dual role for mycobacterial RecO in RecA-dependent homologous recombination and RecA-independent single-strand annealing Journal Article
| Authors: | Gupta, R.; Ryzhikov, M.; Koroleva, O.; Unciuleac, M.; Shuman, S.; Korolev, S.; Glickman, M. S. |
| Article Title: | A dual role for mycobacterial RecO in RecA-dependent homologous recombination and RecA-independent single-strand annealing |
| Abstract: | Mycobacteria have two genetically distinct pathways for the homology-directed repair of DNA double-strand breaks: homologous recombination (HR) and single-strand annealing (SSA). HR is abolished by deletion of RecA and reduced in the absence of the AdnAB helicase/nuclease. By contrast, SSA is RecA-independent and requires RecBCD. Here we examine the function of RecO in mycobacterial DNA recombination and repair. Loss of RecO elicits hypersensitivity to DNA damaging agents similar to that caused by deletion of RecA. We show that RecO participates in RecA-dependent HR in a pathway parallel to the AdnAB pathway. We also find that RecO plays a role in the RecAindependent SSA pathway. The mycobacterial RecO protein displays a zinc-dependent DNA binding activity in vitro and accelerates the annealing of SSB-coated single-stranded DNA. These findings establish a role for RecO in two pathways of mycobacterial DNA double-strand break repair and suggest an in vivo function for the DNA annealing activity of RecO proteins, thereby underscoring their similarity to eukaryal Rad52. © The Author(s) 2013. Published by Oxford University Press. |
| Keywords: | controlled study; unclassified drug; gene deletion; dna-binding proteins; nonhuman; molecular genetics; binding affinity; protein function; dna recombination; dna damage; homologous recombination; dna repair; in vivo study; in vitro study; bacterial strain; strain difference; dna strand breakage; bacterial protein; bacterial proteins; double stranded dna; eukaryota; helicase; single stranded dna; dna, single-stranded; zinc; dna binding; bacterial dna; corynebacterineae; dna helicases; mycobacterium smegmatis; microbial viability; exodeoxyribonuclease v; reca protein; single strand annealing; deoxyribonucleases; rec a recombinases; recombinant dna; recombinational dna repair; recombination repair; adnab helicase; reco protein |
| Journal Title: | Nucleic Acids Research |
| Volume: | 41 |
| Issue: | 4 |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Date Published: | 2013-02-01 |
| Start Page: | 2284 |
| End Page: | 2295 |
| Language: | English |
| PROVIDER: | scopus |
| PMCID: | PMC3575820 |
| PUBMED: | 23295671 |
| DOI: | 10.1093/nar/gks1298 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 1 May 2013" - "CODEN: NARHA" - ":doi 10.1093/nar/gks1298" - "Source: Scopus" |
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