Growth of heterotopic LNCap prostate cancer tumor in nude mice is not affected by dietary calcium Journal Article


Authors: Balaji, K C; Huryk, R. F.; Verhulst, S.; Fair, W. R.
Article Title: Growth of heterotopic LNCap prostate cancer tumor in nude mice is not affected by dietary calcium
Abstract: BACKGROUND. We attempted to provide experimental evidence linking increased dietary calcium to progression of prostate cancer, as suggested by some epidemiological studies, using a heterotopic prostate cancer nude mice model. METHODS. Twenty heterotopic LNCaP prostate cancer tumor bearing nude mice were randomly assigned to one of the four groups: (I) high fat/low calcium diet, (II) high fat and high calcium diet, (III) high fat diet fortified with Vitamin D3, and (IV) high fat and high calcium diet fortified with Vitamin D3. In addition to weekly animal weights and tumor size measurements, the serum prostate specific antigen (PSA), 25-hydroxy Vitamin D3, calcium, phosphorus, total protein, albumin (to account for bound calcium) [1], and serum alkaline phosphatase (a measure of bone loss) [2] were determined at the termination of experiments. RESULTS. Although the serum calcium and 25-hydroxy Vitamin D3 were significantly higher in groups III and IV compared to groups I and II (P < 0.05), there was no significant difference between the tumor growth rates, final tumor weights (P = 0.9), and the serum PSA levels (P = 0.94) between the four groups. CONCLUSIONS. The results suggest that dietary calcium does not significantly affect the growth of heterotopic LNCaP prostate cancer in nude mice. © 2001 Wiley-Liss, Inc.
Keywords: controlled study; cancer growth; nonhuman; adenocarcinoma; prostate specific antigen; blood proteins; animal cell; mouse; animals; mice; cell division; tumor volume; animal experiment; animal model; body weight; calcium; calcium blood level; prostate cancer; prostate-specific antigen; prostatic neoplasms; alkaline phosphatase; diet; albumin; nude mouse; mice, nude; disease progression; vitamin d; cancer cell; measurement; neoplasms, hormone-dependent; growth rate; fat; random allocation; phosphorus; calcium intake; cholecalciferol; serum albumin; colecalciferol; lncap; prostate specific antigen (psa); dietary fats; calcifediol; humans; male; priority journal; article; calcium, dietary
Journal Title: Prostate
Volume: 48
Issue: 4
ISSN: 0270-4137
Publisher: John Wiley & Sons  
Date Published: 2001-09-01
Start Page: 265
End Page: 273
Language: English
DOI: 10.1002/pros.1106
PUBMED: 11536306
PROVIDER: scopus
DOI/URL:
Notes: Export Date: 21 May 2015 -- Source: Scopus
Altmetric Score
MSK Authors
  1. William R Fair
    199 Fair
  2. Robert   Huryk
    19 Huryk
  3. K C Balaji
    7 Balaji