Influence of dietary calcium and vitamin D on diet-induced epithelial cell hyperproliferation in mice Journal Article
| Authors: | Xue, L.; Lipkin, M.; Newmark, H.; Wang, J. |
| Article Title: | Influence of dietary calcium and vitamin D on diet-induced epithelial cell hyperproliferation in mice |
| Abstract: | Background: Previous epidemiologic and laboratory studies, including some from our own laboratory, have suggested that a high-fat diet increases risk of cancer development in the pancreas, prostate, colon, and breast and that carcinogenesis in some of these organs may be influenced by alterations in dietary calcium and vitamin D. In this study, we sought to investigate the effect of added dietary calcium or vitamin D on the development of epithelial cell hyperproliferation induced by a Western-style diet in the exocrine pancreas, prostate, and mammary gland of mice. Methods: Four-week-old C57BL/6J mice were given either a control diet (American Institute of Nutrition [AIN]-76A), a Western-style diet (containing reduced calcium and vitamin D and the fat level of the average human Western diet), or a putative chemopreventive diet (a Western-style diet with the addition of dietary calcium and vitamin D). Nine weeks after dietary intervention, osmotic pumps were implanted in the mice to provide 3 days of bromodeoxyuridine (BrdU) infusion. All P values are two-sided. Results: Mice on the Western-style diet had statistically significant increases in BrdU-labeling indices of epithelial cells in the interlobular (P = .015) and intralobular (P = .012) ducts and centroacinar cells (P = .001) of the pancreatic duct system, the dorsal lobe of the prostate (P = .045), and the terminal ducts of the mammary gland (P = .032), compared with mice in the respective control diet groups. Adding dietary calcium and vitamin D markedly suppressed the Western-style diet-induced hyperproliferation of epithelial cells in those tissues (P = .001-.033). Conclusions: This study confirms previous findings that a Western-style diet produces hyperproliferation of epithelial cells in several organs and that the changes can be prevented by increasing dietary calcium and vitamin D alone. |
| Keywords: | immunohistochemistry; controlled study; cancer risk; nonhuman; pancreas; cell proliferation; animal cell; mouse; animals; mice; animal tissue; cell division; cancer prevention; calcium; mice, inbred c57bl; prostate; tissue distribution; vitamin d; prostate epithelium; epithelium cell; epithelial cells; dietary intake; mammary glands, animal; vitamin intake; fat intake; pancreas cell; male; female; priority journal; article; calcium, dietary; bromodeoxycytidine |
| Journal Title: | JNCI: Journal of the National Cancer Institute |
| Volume: | 91 |
| Issue: | 2 |
| ISSN: | 0027-8874 |
| Publisher: | Oxford University Press |
| Date Published: | 1999-01-20 |
| Start Page: | 176 |
| End Page: | 181 |
| Language: | English |
| PUBMED: | 9923860 |
| PROVIDER: | scopus |
| DOI: | 10.1093/jnci/91.2.176 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
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