Differential in vivo internalization of MOR-I and MOR-IC by morphine Journal Article
| Authors: | Abbadie, C.; Pasternak, G. W. |
| Article Title: | Differential in vivo internalization of MOR-I and MOR-IC by morphine |
| Abstract: | The mu opioid receptor MOR-I is internalized by many mu agonists, but not morphine. To see whether differences in the intracellular carboxy terminus influences internalization, we examined internalization of a splice variant of the mu opioid receptor, MOR-IC, in the lateral septum of the mouse in vivo. Following intracerebroventricular (i.c.v.) saline treatment, MOR-IC-like immunoreactivity (LI) within neurons in naive mice was found predominantly in clusters close to the plasma membrane. Following either intracerebroventricular [D-Ala2, MePhe4,Gly(ol)5]enkephalin (DAMGO) or morphine, MOR-IC-LI clustered into endosomes in the cytoplasm. This effect was suppressed by prior administration of the opioid antagonist naloxone. In contrast, only DAMGO, and not morphine, internalized MOR-I-LI. These results illustrate differences in internalization between two MOR-I variants that have alternative splicing at the COOH terminus. © 2001 Lippincott Williams & Wilkins. |
| Keywords: | immunohistochemistry; controlled study; protein expression; unclassified drug; nonhuman; animal cell; mouse; animals; mice; animal tissue; carboxy terminal sequence; animal experiment; neurons; immunoreactivity; central nervous system; drug receptor binding; protein transport; alternative splicing; alternative rna splicing; cytoplasm; protein structure, tertiary; morphine; endocytosis; protein variant; mu opiate receptor; mu opiate receptor 1c; receptors, opioid, mu; protein isoforms; internalization; naloxone; mu 1 opiate receptor; endosome; opioid receptor; opiate antagonist; enkephalin, ala(2)-mephe(4)-gly(5)-; enkephalin[2 dextro alanine 4 methylphenylalanine 5 glycine]; morphine addiction; splice variant; male; priority journal; article; lateral septum; mopic; mor-ic; septal nuclei |
| Journal Title: | NeuroReport |
| Volume: | 12 |
| Issue: | 14 |
| ISSN: | 0959-4965 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 2001-10-08 |
| Start Page: | 3069 |
| End Page: | 3072 |
| Language: | English |
| PUBMED: | 11568638 |
| PROVIDER: | scopus |
| DOI: | 10.1097/00001756-200110080-00017 |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
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