Differentiation between unlabeled and very-low-level fully 15N, 13C-labeled nucleotides for resonance assignments in nucleic acids Journal Article
| Authors: | Phan, A. T.; Patel, D. J. |
| Article Title: | Differentiation between unlabeled and very-low-level fully 15N, 13C-labeled nucleotides for resonance assignments in nucleic acids |
| Abstract: | Based on different characteristics between unlabeled and fully 15N,13C-labeled nucleotides, we develop a method for unambiguous resonance assignments in nucleic acids following site-specific fully 15N,13C isotope incorporation at very low levels1. The J-couplings between heteronuclei provide for distinction between the NMR signals of the fully labeled nucleotides and those of the natural abundance nucleotides. The method is demonstrated for DNA oligonucleotides2, in the dimeric G-quadruplex [d(GGGTTCAGG)]2 and in the 22-nucleotide human telomeric fragment d[AG3(TTAG3)3]. We expect this approach to be useful for selective monitoring of important functional domains and of their interactions in large nucleic acids. |
| Keywords: | controlled study; methodology; monitoring; telomere; correlation analysis; chemistry; dna; isotope labeling; guanosine; nucleic acids; chemical structure; oligonucleotide; signal detection; nuclear magnetic resonance; nitrogen; carbon; carbon isotopes; nitrogen isotopes; nucleotide; magnetism; magnetics; nucleic acid; carbon nuclear magnetic resonance; purine; oligodeoxyribonucleotides; nucleic acid structure; nuclear magnetic resonance, biomolecular; nucleotides; chemical interaction; carbon 13; quadruplex dna; nitrogen 15; priority journal; article; oligodeoxyribonucleotide; low isotopic abundance; low-level fully labeled nucleotides; model-independent assignments; site-specific enrichment |
| Journal Title: | Journal of Biomolecular NMR |
| Volume: | 23 |
| Issue: | 4 |
| ISSN: | 0925-2738 |
| Publisher: | Springer |
| Date Published: | 2002-08-01 |
| Start Page: | 257 |
| End Page: | 262 |
| Language: | English |
| DOI: | 10.1023/a:1020277223482 |
| PUBMED: | 12398346 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
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