| Abstract: |
Defects of DNA repair systems in cutaneous tumours are related to DNA mismatch repair genes (MLH1, MSH2, PMS1, PMS2) and Ku/70/80 genes involved in double- strand repair. In this study we investigated the statistical relationship between these systems and DNA-ploidy-related parameters in 19 naevus cell naevi, 23 lentigos maligna, 76 primary melanomas and 31 melanoma metastases, applying the correlation coefficient according to Spearman. In naevi significant correlations were found between Ku/70/80 gene expression and some ploidy-related parameters. In lentigos, additionally, some significant correlations between the expression of DNA mismatch repair genes were found. Similar results were demonstrated for primary melanomas. In metastases no one significant correlation between DNA mistmatch repair genes and Ku-genes was present. We postulate that DNA mismatch repair genes and Ku/70/80 genes are functionally independent and that some of them are able to influence ploidy-related parameters. |
| Keywords: |
immunohistochemistry; adult; aged; aged, 80 and over; middle aged; carrier protein; dna binding protein; oncoprotein; major clinical study; genetics; dna-binding proteins; proto-oncogene proteins; cancer growth; lymph node metastasis; lymphatic metastasis; metabolism; dna repair; melanoma; metastasis; gene expression; nevus; skin neoplasms; malignant lentigo; hutchinson's melanotic freckle; nuclear protein; neoplasm proteins; pathology; nuclear proteins; skin tumor; statistical analysis; dna; melanocytosis; mismatch repair; carrier proteins; tumor protein; dna, neoplasm; dna repair enzymes; cell count; helicase; ku antigen; polydeoxyribonucleotide synthase; adenosine triphosphatase; mlh1 protein, human; protein msh2; msh2 protein, human; pms2 protein, human; adenosine triphosphatases; muts homolog 2 protein; skin metastasis; dna helicases; base mispairing; base pair mismatch; antigens, nuclear; cell nucleus antigen; correlation function; nevus cell; ploidy; ploidies; humans; human; male; female; article; ploidy status; dna-mismatch repair; ku-proteins; pms1 protein, human; xrcc5 protein, human; image cytometry
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