Quantitative analysis of Ku70 and Ku80 mrna gene expression in melanoma brain metastases. correlation with immunohistochemistry and in situ hybridization Journal Article

  


Authors: Korabiowska, M.; König, F.; Schlott, T.; Slotty, P.; Romeike, B.; Cordon-Cardo, C.; Brinck, U.
Article Title: Quantitative analysis of Ku70 and Ku80 mrna gene expression in melanoma brain metastases. correlation with immunohistochemistry and in situ hybridization
Abstract: Background: Altered expression and prognostic significance of DNA double-strand repair genes Ku70 and Ku80 has been shown by our research group for malignant melanomas. High genomic instability known for melanoma brain metastases stimulated us to analyze Ku70 and Ku80 expression in melanoma brain metastases. Materials and Methods: Quantitative evaluation of mRNA Ku70 and Ku80 expression was performed in 13 melanoma brain metastases. Immunohistochemistry and in situ hybridization for Ku70 and Ku80 were applied to 34 metastatic tumours. Results: Quantitative analysis of Ku70 mRNA expression demonstrated values between 0.01 and 0.33. Ku80 mRNA expression ranged between 0.001 and 0.54. Immunohistochemistry demonstrated Ku70 and Ku80 expression in 34 and in 25 metastatic tumours, respectively. In situ hybridization detected Ku70 expression in 19/34 and Ku80 expression in 13/34 metastatic tumours. Correlation between Ku70 and Ku80 expression in melanoma brain metastases was lost. Conclusion: Ku70 and especially Ku80 expression is altered in melanoma brain metastases and corresponds with the high genomic instability of these lesions.
Keywords: ku70; ku80; brain tumours; dnarepair; melanoma brain metastases
Journal Title: Cancer Genomics and Proteomics
Volume: 1
Issue: 3
ISSN: 1109-6535
Publisher: The International Institute of Anticancer Research  
Date Published: 2004-05-01
Start Page: 225
End Page: 230
Language: English
PROVIDER: scopus
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84873334446&partnerID=40&md5=a324ec99e1e1a767525f455e9a3674fa
Notes: Cancer Genomics Proteomics -- Export Date: 16 June 2014 -- Source: Scopus