A correlative optical microscopy and scanning electron microscopy approach to locating nanoparticles in brain tumors Journal Article

   

Authors:	Kempen, P. J.; Kircher, M. F.; De La Zerda, A.; Zavaleta, C. L.; Jokerst, J. V.; Mellinghoff, I. K.; Gambhir, S. S.; Sinclair, R.
Article Title:	A correlative optical microscopy and scanning electron microscopy approach to locating nanoparticles in brain tumors
Abstract:	The growing use of nanoparticles in biomedical applications, including cancer diagnosis and treatment, demands the capability to exactly locate them within complex biological systems. In this work a correlative optical and scanning electron microscopy technique was developed to locate and observe multi-modal gold core nanoparticle accumulation in brain tumor models. Entire brain sections from mice containing orthotopic brain tumors injected intravenously with nanoparticles were imaged using both optical microscopy to identify the brain tumor, and scanning electron microscopy to identify the individual nanoparticles. Gold-based nanoparticles were readily identified in the scanning electron microscope using backscattered electron imaging as bright spots against a darker background. This information was then correlated to determine the exact location of the nanoparticles within the brain tissue. The nanoparticles were located only in areas that contained tumor cells, and not in the surrounding healthy brain tissue. This correlative technique provides a powerful method to relate the macro- and micro-scale features visible in light microscopy with the nanoscale features resolvable in scanning electron microscopy.
Keywords:	cancer diagnosis; electron microscopy; brain; tumors; diagnosis; nanoparticles; electrons; diseases; tissue; nanotechnology; medical applications; gold; scanning electron microscopy; biomedical applications; optical microscopy; correlative microscopy; optical data storage; backscattered electron imaging; brain tumor model; complex biological systems; correlative microscopies; individual nanoparticles; nanoscale features
Journal Title:	Micron
Volume:	68
ISSN:	0968-4328
Publisher:	Pergamon-Elsevier Science Ltd
Date Published:	2015-01-01
Start Page:	70
End Page:	76
Language:	English
DOI:	10.1016/j.micron.2014.09.004
PROVIDER:	scopus
PMCID:	PMC4262686
PUBMED:	25464144
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84910649983&partnerID=40&md5=42f5f1004130e34423074a616dcdbaba
Notes:	Export Date: 1 December 2014 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/64982