Independent recruitment in vivo by Gal4 of two complexes required for transcription Journal Article
| Authors: | Bryant, G. O.; Ptashne, M. |
| Article Title: | Independent recruitment in vivo by Gal4 of two complexes required for transcription |
| Abstract: | We use a modified form of ChIP to analyze the recruitment of seven sets of proteins to the yeast GAL genes upon induction. We resolve three stages of recruitment: first SAGA, then Mediator, and finally Pol II along with four other proteins (including TBP) bind the promoter. In a strain lacking SAGA, Mediator is recruited with a time course indistinguishable from that observed in wild-type cells. Our results are consistent with the notion that a single species of activator, Gal4, separately contacts, and thereby directly recruits, SAGA and Mediator. |
| Keywords: | unclassified drug; promoter region; protein analysis; cells, cultured; transcription initiation; protein; genetic transcription; in vivo study; wild type; transcription factors; gene expression regulation; binding protein; binding sites; trans-activators; saccharomyces cerevisiae proteins; protein determination; rna polymerase ii; gene expression regulation, fungal; macromolecular substances; reaction time; yeasts; cell; mediator; galactose; promoter regions (genetics); genes, regulator; trans-activation (genetics); transcription factor saga; article; genetic strain; galactose 4; raffinose |
| Journal Title: | Molecular Cell |
| Volume: | 11 |
| Issue: | 5 |
| ISSN: | 1097-2765 |
| Publisher: | Cell Press |
| Date Published: | 2003-05-01 |
| Start Page: | 1301 |
| End Page: | 1309 |
| Language: | English |
| DOI: | 10.1016/s1097-2765(03)00144-8 |
| PUBMED: | 12769853 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Source: Scopus |
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