Activation of the Gal1 gene of yeast by pairs of 'non-classical' activators Journal Article
| Authors: | Cheng, J. X.; Gandolfi, M.; Ptashne, M. |
| Article Title: | Activation of the Gal1 gene of yeast by pairs of 'non-classical' activators |
| Abstract: | Eukaryotic transcriptional activators work by recruiting to DNA the transcriptional machinery, including protein complexes required for chromatin modification, transcription initiation, and elongation [1, 2]. Which of these complexes must be directly recruited to trigger transcription? We test various "non-classical" transcription activators (comprising a component of the transcriptional machinery fused to a DNA binding domain) for their abilities to activate transcription of a chromosomally integrated reporter in yeast. Among these newly constructed fusion proteins, none efficiently activated transcription when working on its own. However, in several instances transcription was activated by a pair of such fusion proteins tethered to adjacent sites on DNA. In each of these cases, one fusion protein bore a component of the SAGA complex, and the other bore a component of the Mediator complex. Transcription was also activated by certain tripartite fusion proteins comprising a Mediator and a SAGA component fused to a DNA binding domain. The results are consistent with the finding [3] that the classical activator Gal4, working at the GAL1 promoter, activates transcription by (at least in part) independently recruiting SAGA and Mediator. |
| Keywords: | dna binding protein; promoter region; genetics; dna-binding proteins; comparative study; metabolism; transcription factor; physiology; transcription factors; gene expression regulation; hybrid protein; recombinant fusion proteins; eukaryota; transactivation; plasmid; reporter gene; plasmids; genes, reporter; yeast; saccharomyces cerevisiae proteins; saccharomyces cerevisiae protein; fungal protein; gene expression regulation, fungal; yeasts; fungal proteins; prokaryota; promoter regions (genetics); trans-activation (genetics); article; gal4 protein, s cerevisiae |
| Journal Title: | Current Biology |
| Volume: | 14 |
| Issue: | 18 |
| ISSN: | 0960-9822 |
| Publisher: | Cell Press |
| Date Published: | 2004-09-21 |
| Start Page: | 1675 |
| End Page: | 1679 |
| Language: | English |
| DOI: | 10.1016/j.cub.2004.09.025 |
| PROVIDER: | scopus |
| PUBMED: | 15380071 |
| DOI/URL: | |
| Notes: | Curr. Biol. -- Cited By (since 1996):8 -- Export Date: 16 June 2014 -- CODEN: CUBLE -- Source: Scopus |
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