Activation of transcription in vitro by recruitment of the yeast RNA polymerase II holoenzyme Journal Article
| Authors: | Gaudreau, L.; Adam, M.; Ptashne, M. |
| Article Title: | Activation of transcription in vitro by recruitment of the yeast RNA polymerase II holoenzyme |
| Abstract: | It has been argued that many transcriptional activators work by "recruitment," that is, by helping the transcriptional machinery bind stably to DNA. We demonstrate here a realization of a strong prediction of this idea in an in vitro transcription reaction performed with purified yeast RNA polymerase II holoenzyme and a classical transcriptional activator. We show that the level of transcription reached by the activator working on low concentrations of holoenzyme can also be reached in the absence of activator by raising the holoenzyme concentration, and that under that condition the activator has no further stimulatory effect. We also show, in agreement with another prediction of the recruitment model, that in a reaction using a holoenzyme purified from cells bearing the "P" mutation, transcription is stimulated by a DNA-tethered peptide that binds the mutant holoenzyme component Gal1 1P but that lacks a classical activating region. |
| Keywords: | promoter region; genetics; metabolism; transcription factor; enzymology; physiology; transcription factors; gene expression regulation; transactivation; reporter gene; genes, reporter; yeast; saccharomyces cerevisiae proteins; saccharomyces cerevisiae protein; rna polymerase ii; gene expression regulation, fungal; coenzyme; coenzymes; gene expression regulation, enzymologic; yeasts; promoter regions (genetics); trans-activation (genetics); transcription factors, tfii; article; gal4 protein, s cerevisiae; transcription factor ii; transcription factor tfiie |
| Journal Title: | Molecular Cell |
| Volume: | 1 |
| Issue: | 6 |
| ISSN: | 1097-2765 |
| Publisher: | Cell Press |
| Date Published: | 1998-05-01 |
| Start Page: | 913 |
| End Page: | 916 |
| Language: | English |
| PUBMED: | 9660974 |
| PROVIDER: | scopus |
| DOI: | 10.1016/S1097-2765(00)80090-8 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
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