Transcriptional activation by artificial recruitment in mammalian cells Journal Article

Authors: Nevado, J.; Gaudreau, L.; Adam, M.; Ptashne, M.
Article Title: Transcriptional activation by artificial recruitment in mammalian cells
Abstract: We show that the typical 'nonclassical' activator, which comprises a fusion protein bearing a component of the transcriptional machinery fused to a DNA-binding domain, activates transcription in mammalian cells only weakly when tested with an array of promoters. However, as found in analogous 'artificial recruitment' experiments performed in yeast, these activators work synergistically with 'classical' activators. The effect of the classical activator in such experiments requires that it be tethered to DNA, a requirement that cannot be overcome by expression of that classical activator at high levels. The effect of the one nonclassical activator that does elicit significant levels of transcription when working alone (i.e., that bearing TATA box-binding protein) is strongly influenced by promoter architecture. The results, consistent with those of analogous experiments in yeast [see the accompanying paper: Gaudreau, L., Keaveney, M., Nevado, J., Zaman, Z., Bryant, G. O., Struhl, K. and Ptashne, M. (1999) Proc. Natl. Acad. Sci. USA 96, 2668-2673], suggest that classical activators, presumably by virtue of their abilities to interact with multiple targets, have a functional flexibility that nonclassical activators lack.
Keywords: controlled study; human cell; promoter region; conference paper; protein domain; genetic transcription; transcription, genetic; hela cell; hela cells; transcription factors; gene activation; dna; hybrid protein; dna binding; mammal cell; trans-activation (genetics); tata binding protein; humans; human; priority journal
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 96
Issue: 6
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1999-03-16
Start Page: 2674
End Page: 2677
Language: English
DOI: 10.1073/pnas.96.6.2674
PUBMED: 10077569
PROVIDER: scopus
Notes: Conference Paper -- Export Date: 16 August 2016 -- Source: Scopus
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MSK Authors
  1. Mark Ptashne
    61 Ptashne
  2. Julian   Nevado
    3 Nevado
  3. Maryse   Adam
    2 Adam