Telomere looping permits repression "at a distance" in yeast Journal Article
| Authors: | Zaman, Z.; Heid, C.; Ptashne, M. |
| Article Title: | Telomere looping permits repression "at a distance" in yeast |
| Abstract: | In yeast, unlike in higher eukaryotes, transcriptional activators and repressors do not normally work when bound to DNA at large distances (over 500 base pairs) [1] from the gene and, in particular, when positioned downstream of the gene [2, 3]. This restriction is relieved for a transcriptional activator if a gene bearing an activator binding site is placed near a yeast telomere [4]. The explanation proposed is that the folded structure found at the telomere helps appose the DNA-bound activator with proteins binding to the promoter so that recruitment of the transcriptional machinery can be effected "at a distance" [4]. Here, we show that a repressor, Tup1, works when tethered to DNA downstream of, and some 1.5-kb from, the gene when the construct is placed near a yeast telomere. The effect, observed with activated as well as basal transcription, is eliminated by deletion of Sir3. These and other results indicate that DNA-tethered Tup1 represses by interacting with some component of the transcriptional machinery binding to the promoter, an interaction that is facilitated by the preformed loop at the telomere. |
| Keywords: | genetics; telomere; saccharomyces cerevisiae; eukaryota; chromatin; nucleotide sequence; base sequence; serodiagnosis; dna primers; primer dna; precipitin tests; article; myxogastria; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Current Biology |
| Volume: | 12 |
| Issue: | 11 |
| ISSN: | 0960-9822 |
| Publisher: | Cell Press |
| Date Published: | 2002-06-04 |
| Start Page: | 930 |
| End Page: | 933 |
| Language: | English |
| DOI: | 10.1016/s0960-9822(02)00865-5 |
| PUBMED: | 12062058 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 23 September 2016 -- Source: Scopus |
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