HSP90/70 chaperones are required for rapid nucleosome removal upon induction of the GAL genes of yeast Journal Article

   


Authors: Floer, M.; Bryant, G. O.; Ptashne, M.
Article Title: HSP90/70 chaperones are required for rapid nucleosome removal upon induction of the GAL genes of yeast
Abstract: Induction of transcription of the GAL genes of yeast by galactose is a multistep process: Galactose frees the activator Gal4 of its inhibitor, Gal80, allowing Gal4 to recruit proteins required to transcribe the GAL genes. Here, we show that deletion of components of either the HSP90 or the HSP70 chaperone machinery delays this induction. This delay remains when the galactose-signaling pathway is bypassed, and it cannot be explained by a chaperone requirement for DNA binding by Gal4. Removal of promoter-bound nucleosomes is delayed in a chaperone mutant, and our findings suggest an involvement of HSP90 and HSP70 in this early step in gene induction. © 2008 by The National Academy of Sciences of the USA.
Keywords: promoter region; gene deletion; genetics; nonhuman; comparative study; mutant protein; metabolism; reverse transcription polymerase chain reaction; gene expression; genetic transcription; physiology; gene expression regulation; reverse transcriptase polymerase chain reaction; saccharomyces cerevisiae; chromatin immunoprecipitation; recombinant proteins; recombinant protein; transcription; heat shock protein 90; hsp90 heat-shock proteins; heat shock protein 70; gene induction; yeast; saccharomyces cerevisiae proteins; dna primers; primer dna; saccharomyces cerevisiae protein; dna binding; adenosine triphosphatase; adenosine triphosphatases; gene expression regulation, fungal; hsp70 heat-shock proteins; chaperone; nucleosome; nucleosomes; activation; recruitment; promoter; galactokinase; gal1 protein, s cerevisiae; gal4; galactose; ssa1 protein, s cerevisiae
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 105
Issue: 8
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 2008-02-26
Start Page: 2975
End Page: 2980
Language: English
DOI: 10.1073/pnas.0800053105
PUBMED: 18287040
PROVIDER: scopus
PMCID: PMC2268570
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-42949109267&partnerID=40&md5=48409f6980359181a1f2c61aa0a3b230
Notes: --- - "Cited By (since 1996): 20" - "Export Date: 17 November 2011" - "CODEN: PNASA" - "Source: Scopus"
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MSK Authors
  1. Mark Ptashne
    61 Ptashne
  2. Gene Bryant
    14 Bryant
  3. Monique Floer
    5 Floer
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