A target essential for the activity of a nonacidic yeast transcriptional activator Journal Article

   


Authors: Lu, Z.; Ansari, A. Z.; Lu, X.; Ogirala, A.; Ptashne, M.
Article Title: A target essential for the activity of a nonacidic yeast transcriptional activator
Abstract: P201 is a short (eight-residue) nonacidic peptide that comprises a strong transcriptional activating region when tethered to DNA in yeast. Here we identify the mediator protein Gal11 as an essential target of P201. Deletion of Gal11, which modestly decreases activation elicited by the typical acidic yeast activator, abolishes activation by DNA-tethered P201, A point mutation in Gal11, which has no effect on other Gal11 functions, also greatly diminishes activation by DNA-tethered P201, P201 binds to a fragment of Gal11 in vivo and in vitro, and the interaction is diminished by mutations in either component that decrease activation in vivo. P201, unlike the typical yeast acidic activating region, does not work in mammalian cells, which is consistent with the notion that the unique target of P201 (Gal11) is absent from mammalian cells.
Keywords: unclassified drug; sequence deletion; nonhuman; protein function; protein analysis; mammalia; transcription initiation; protein; protein binding; genotype; protein interaction; protein purification; saccharomyces cerevisiae; trans-activators; yeast; saccharomyces cerevisiae proteins; point mutation; oligopeptides; fungal proteins; priority journal; article; protein gal 11; protein p201
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 99
Issue: 13
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 2002-06-25
Start Page: 8591
End Page: 8596
Language: English
DOI: 10.1073/pnas.092263499
PUBMED: 12084920
PROVIDER: scopus
PMCID: PMC124323
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-0037173061&partnerID=40&md5=db7d349d570f6f7075f3819680abb424
Notes: Export Date: 14 November 2014 -- Source: Scopus
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MSK Authors
  1. Mark Ptashne
    61 Ptashne
  2. Zhen Lu
    2 Lu
  3. Aseem Z Ansari
    10 Ansari
  4. Anuja Ogirala
    13 Ogirala
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