N-glycoprotein surfaceomes of four developmentally distinct mouse cell types Journal Article
| Authors: | Kropp, E. M.; Bhattacharya, S.; Waas, M.; Chuppa, S. L.; Hadjantonakis, A. K.; Boheler, K. R.; Gundry, R. L. |
| Article Title: | N-glycoprotein surfaceomes of four developmentally distinct mouse cell types |
| Abstract: | Purpose: Detailed knowledge of cell surface proteins present during early embryonic development remains limited for most cell lineages. Due to the relevance of cell surface proteins in their functional roles controlling cell signaling and their utility as accessible, nongenetic markers for cell identification and sorting, the goal of this study was to provide new information regarding the cell surface proteins present during early mouse embryonic development. Experimental Design: Using the cell surface capture technology, the cell surface N-glycoproteomes of three cell lines and one in vitro differentiated cell type representing distinct cell fates and stages in mouse embryogenesis were assessed. Results: Altogether, more than 600 cell surface N-glycoproteins were identified represented by >5500 N-glycopeptides. Conclusions and Clinical Relevance: The development of new, informative cell surface markers for the reliable identification and isolation of functionally defined subsets of cells from early developmental stages will advance the use of stem cell technologies for mechanistic developmental studies, including disease modeling and drug discovery. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| Keywords: | controlled study; unclassified drug; nonhuman; biomarkers; protein analysis; animal cell; mouse; embryo; cell fate; embryo development; cell differentiation; in vitro study; proteomics; cell type; fibroblast; ephrin receptor a2; cd24 antigen; heart muscle cell; glycoproteins; cell surface protein; cardiovascular; plasma membrane; priority journal; article; glycoproteomics; activated leukocyte cell adhesion molecule; cd276 antigen; cell surface n glycoprotein; multidrug resistance associated protein 1; multidrug resistance associated protein 4; protein ano6; protein atp1b1; protein atp1b3; protein bcam; protein bst2; protein cadm1; protein clcn2; protein cldnd1; protein enpep; protein epha2; protein f11r; protein gpc3; protein itfg3; protein lnpep; protein lpar4; protein mpzl1; protein ncam1; protein nradd; protein pdgfra; protein pecam1; protein piezo1; embryonic fibroblast; embryonic stem cell derived cardiomyocyte; extraembryonic endoderm cell |
| Journal Title: | Proteomics Clinical Applications |
| Volume: | 8 |
| Issue: | 7-8 |
| ISSN: | 1862-8346 |
| Publisher: | Wiley V C H Verlag Gmbh |
| Date Published: | 2014-08-01 |
| Start Page: | 603 |
| End Page: | 609 |
| Language: | English |
| DOI: | 10.1002/prca.201400021 |
| PROVIDER: | scopus |
| PMCID: | PMC4151168 |
| PUBMED: | 24920426 |
| DOI/URL: | |
| Notes: | Export Date: 2 September 2014 -- Source: Scopus |
