Human pluripotent stem cells: An emerging model in developmental biology Journal Article
| Authors: | Zhu, Z.; Huangfu, D. |
| Article Title: | Human pluripotent stem cells: An emerging model in developmental biology |
| Abstract: | Developmental biology has long benefited from studies of classic model organisms. Recently, human pluripotent stem cells (hPSCs), including human embryonic stem cells and human induced pluripotent stem cells, have emerged as a new model system that offers unique advantages for developmental studies. Here, we discuss how studies of hPSCs can complement classic approaches using model organisms, and how hPSCs can be used to recapitulate aspects of human embryonic development 'in a dish'. We also summarize some of the recently developed genetic tools that greatly facilitate the interrogation of gene function during hPSC differentiation. With the development of high-throughput screening technologies, hPSCs have the potential to revolutionize gene discovery in mammalian development. © 2013. |
| Keywords: | dna binding protein; review; gene targeting; dna repair; biological model; embryonic stem cell; transcription initiation; small interfering rna; cell fate; developmental biology; embryo development; gene function; morphogenesis; rna interference; cell differentiation; high throughput screening; genetic manipulation; extracellular matrix; disease model; chromosome aberration; progeny; transgene; development; pluripotent stem cell; gene silencing; developmental disorder; genetic screening; mesoderm; transient transfection; short hairpin rna; endoderm; dna cleavage; blastocyst; transgenics; gastrulation; directed differentiation; recombinant growth factor; copy number variation; ectoderm; high-throughput screening; germ layer; regenerative medicine; human pluripotent stem cells; embryoid body; feeder cell; human development |
| Journal Title: | Development |
| Volume: | 140 |
| Issue: | 4 |
| ISSN: | 0950-1991 |
| Publisher: | Company of Biologists |
| Date Published: | 2013-02-01 |
| Start Page: | 705 |
| End Page: | 717 |
| Language: | English |
| DOI: | 10.1242/dev.086165 |
| PROVIDER: | scopus |
| PMCID: | PMC3557771 |
| PUBMED: | 23362344 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 1 March 2013" - "CODEN: DEVPE" - "Source: Scopus" |
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