Feeder-free derivation of neural crest progenitor cells from human pluripotent stem cells Journal Article
| Authors: | Zeltner, N.; Lafaille, F. G.; Fattahi, F.; Studer, L. |
| Article Title: | Feeder-free derivation of neural crest progenitor cells from human pluripotent stem cells |
| Abstract: | Human pluripotent stem cells (hPSCs) have great potential for studying human embryonic development, for modeling human diseases in the dish and as a source of transplantable cells for regenerative applications after disease or accidents. Neural crest (NC) cells are the precursors for a large variety of adult somatic cells, such as cells from the peripheral nervous system and glia, melanocytes and mesenchymal cells. They are a valuable source of cells to study aspects of human embryonic development, including cell fate specification and migration. Further differentiation of NC progenitor cells into terminally differentiated cell types offers the possibility to model human diseases in vitro, investigate disease mechanisms and generate cells for regenerative medicine. This article presents the adaptation of a currently available in vitro differentiation protocol for the derivation of NC cells from hPSCs. This new protocol requires 18 days of differentiation, is feeder-free, easily scalable and highly reproducible among human embryonic stem cell (hESC) lines as well as human induced pluripotent stem cell (hiPSC) lines. Both old and new protocols yield NC cells of equal identity. |
| Keywords: | neural crest; pluripotent stem cells; neuroscience; human embryonic stem cells; neural crest cells; disease modeling; human pluripotent stem cells; in vitro differentiation; issue 87; embryonic stem cells (escs); induced pluripotent stem cells (ipscs); differentiation protocol; peripheral nervous system (pns) |
| Journal Title: | Journal of Visualized Experiments |
| Issue: | 87 |
| ISSN: | 1940-087X |
| Publisher: | MYJoVE Corporation |
| Date Published: | 2014-05-22 |
| Start Page: | e51609 |
| Language: | English |
| DOI: | 10.3791/51609 |
| PROVIDER: | scopus |
| PMCID: | PMC4204793 |
| PUBMED: | 24893703 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 14 February 2017 -- Source: Scopus |
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