Dual-SMAD inhibition/WNT activation-based methods to induce neural crest and derivatives from human pluripotent stem cells Journal Article

  


Authors: Chambers, S. M.; Mica, Y.; Lee, G.; Studer, L.; Tomishima, M. J.
Article Title: Dual-SMAD inhibition/WNT activation-based methods to induce neural crest and derivatives from human pluripotent stem cells
Abstract: The neural crest (NC) is a transient population of multipotent cells giving rise to the peripheral nervous system, skin pigmentation, heart, and facial mesenchyme. The broad cell fate potential of NC makes it an attractive cell fate to derive from human pluripotent stem cells (hPSCs) for exploring embryonic deve lopment,modeling disease, and generating cells for transplantation. Here, we discuss recent publications and methods for efficiently differentiating hPSCs into NC. We also provide methods to direct NC into two different terminal fates: melanocytes and sensory neurons. © Springer Science+Business Media New York 2013.
Keywords: controlled study; human cell; nonhuman; animal cell; mouse; melanocyte; smad protein; transforming growth factor beta; cell fate; cell differentiation; central nervous system; cell density; neural crest; pluripotent stem cell; wnt protein; sensory nerve cell; wnt3 protein; disease modeling; dual smad inhibition; human pluripotent stem cells; protein nodal; activin; human; priority journal; article; peripheral sensory neurons; 6 [[2 [[4 (2,4 dichlorophenyl) 5 (4 methyl 1h imidazol 2 yl) 2 pyrimidinyl]amino]ethyl]amino]nicotinonitrile
Journal Title: Methods in Molecular Biology
Volume: 1307
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2016-01-01
Start Page: 329
End Page: 343
Language: English
DOI: 10.1007/7651_2013_59
PROVIDER: scopus
PUBMED: 24301074
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-84938840735&partnerID=40&md5=255a8cdaf7061c3365e0629bedc9edde
Notes: Chapter in "Human Embryonic Stem Cell Protocols" (ISBN: 978-1-4939-2667-1) -- Export Date: 21 June 2016 -- Source: Scopus
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MSK Authors
  1. Lorenz Studer
    224 Studer
  2. Mark J Tomishima
    45 Tomishima
  3. Stuart M Chambers
    15 Chambers
  4. Yvonne Gruber
    6 Gruber
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